Datasheet

HINT1 Antibody
CATALOG NUMBER: 4815

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Bovine: (100%)
TESTED APPLICATIONS:ELISA, ICC, WB
APPLICATIONS:HINT1 antibody can be used for detection of HINT1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 17-005 - Jurkat Cell Slide
IMMUNOGEN:HINT1 antibody was raised against a 17 amino acid synthetic peptide near the center of the human HINT1.

The immunogen is located within the last 50 amino acids of HINT1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:HINT1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:HINT1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:HINT1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:HINT1 Antibody: HINT, NMAN, PKCI-1, PRKCNH1, HINT, PKCI1, Histidine triad nucleotide-binding protein 1, Protein kinase C inhibitor 1
ACCESSION NO.:NP_005331
PROTEIN GI NO.:4885413
OFFICIAL SYMBOL:HINT1
GENE ID:3094

Background

BACKGROUND:HINT1 Antibody: Histidine triad nucleotide-binding protein 1 (HINT1) is a member of the histidine triad (HIT) protein family, a group of small nucleotide-binding and -hydrolyzing proteins. HINT1 interacts with several diverse proteins and has been suggested to have tumor suppressive activities. HINT1 catalyzes the hydrolysis of adenosine 5'-monophoramidate substrates such as AMP-morpholidate, but its enzymatic function does not appear to play a role in its tumor suppression. Recent experiments demonstrate that HINT1 forms a complex with POSH and JNK in vivo, inhibiting AP-1 activity and the phosphorylation of c-Jun, and this action could contribute to the tumor suppressor activity of HINT1. Other studies raise the possibility of HINT1 as a candidate gene for schizophrenia.
REFERENCES: 1) Lima CD, Klein MG, Weinstein IB, et al. Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins. Proc. Natl. Acad. Sci. USA1996; 93:5357-62.
2) Weiske J and Huber O. Beta-catenin takes a HIT. Cell Cycle2008; 7:1326-31.
3) Wang L, Zhang Y, Li H, et al. Hint1 inhibits growth and activator protein-1 activity in human colon cancer cells. Cancer Res.2007; 67:4700-8.
4) Chen Q, Wang X, O’Neill FA, et al. Is the histidine triad nucleotide-binding protein 1 (HINT1) gene a candidate for schizophrenia? Schizophr. Res.2008; 106:200-7.

For Research Use Only