HIG2 Antibody Cat. No.: 6491

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
IMMUNOGEN: HIG2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human HIG2.

The immunogen is located within the last 50 amino acids of HIG2.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: HIG2 antibody can be used for detection of HIG2 by Western blot at 1 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
SPECIFICITY: At least two isoforms of HIG2 are known to exist; this antibody will detect both isoforms. HIG2 antibody is predicted to not cross-react with HIG1
POSITIVE CONTROL:1) Cat. No. 1203 - A549 Cell Lysate

psi-iconProperties
PURIFICATION:HIG2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:HIG2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:HIG2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:HIGD2A
ALTERNATE NAMES:HIG2 Antibody: RCF1b, HIG1 domain family member 2A, mitochondrial, RCF1 homolog B, RCF1b
ACCESSION NO.:NP_001092256
PROTEIN GI NO.:20270389
GENE ID:192286
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:HIG2 Antibody: HIG1 and HIG2 (Hypoxia-inducible gene 1 and 2, respectively) are known to be induced by hypoxic conditions. HIG2 is induced by hypoxia and by glucose deprivation in cultured cells. In addition, tumor xenografts derived from human cervical cancer cells display increased expression of HIG1 and HIG2 when they are deprived of oxygen. Unlike HIG2, which is ubiquitously expressed and might be an activator and target of the canonical Wnt pathway, the function and the mechanisms underlying its regulation of HIG1 still remained unknown. The putative link between hypoxia and an oncogenic signaling pathway might play an important role in tumorigenesis.
REFERENCES:1) Bedo G, Vargas M, Ferreiro MJ, et al. Characterization of hypoxia induced gene 1: expression during rat central nervous system maturation and evidence of antisense RNA expression. Int. J. Dev. Biol. 2005; 49:431-6
2) Simpson JC, Wellenreuther R, Poustka A, et al. Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. EMBO Rep. 2000; 1:287-92
3) Denko NC, Schindler C, Koong A, et al. Epigenetic regulation of gene expression in cervical cancer cells by the tumor microenvironment. Clin. Cancer Res. 2000; 6:480-7
4) Gimm T, Wiese M, Teschemacher B, et al. Hypoxia-inducible protein 2 is a novel lipid droplet protein and a specific target gene of hypoxia-inducible factor-1. FASEB J. 2010; 24:4443-58.

ANTIBODIES FOR RESEARCH USE ONLY.

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