Datasheet

HHATL Antibody
CATALOG NUMBER: 6787

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:HHATL antibody can be used for detection of HHATL by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
 2) Cat. No. 12-701 - Human Skin Tissue Slide
IMMUNOGEN:HHATL antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human HHATL.

The immunogen is located within the first 50 amino acids of HHATL.
HOST SPECIES:Rabbit

Properties

PURIFICATION:HHATL Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:HHATL Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:HHATL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:HHATL Antibody: GUP1, OACT3, C3orf3, MBOAT3, MSTP002, GUP1, KIAA1173, Protein-cysteine N-palmitoyltransferase HHAT-like protein, Hedgehog acyltransferase-like protein
ACCESSION NO.:NP_065758
PROTEIN GI NO.:239582769
OFFICIAL SYMBOL:HHATL
GENE ID:57467

Background

BACKGROUND:HHATL Antibody: The Protein-cysteine N-palmitoyltransferase HHAT-like protein (HHATL), also known at the mammalian glycerol uptake/transporter 1 (Gup1) is a homolog of the S. cerevisiae Gup1. It is an endoplasmic reticulum (ER) membrane protein that co-localizes with the mammalian hedgehog acyltransferse Skn and interferes with its N-terminal palmitoylation of Sonic hedgehog (SHH), suggesting that HHATL acts as a negative regulator for N-terminal palmitoylation of SHH. HHATL is highly expressed in normal skin, but becomes down-regulated in skin squamous cell carcinoma, suggesting it may play a role in its development.
REFERENCES: 1) Abe Y, Kita Y, and Niikura T. Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-palmitoylation of Sonic hedgehog. FEBS J. 2008; 275:318-31.
2) Zhang SQ, Tian X, Luo YW, et al. Expression, clinical and pathological significance of KIAA1173 gene in skin squamous cell carcinoma. Zhonghua Yi Xue Za Zhi 2010; 90:1243-6.

For Research Use Only