The ED50 was determined by the dose - dependent stimulation of the proliferation of monkey 4MBr - 5 cells was found to be in the range of 20.0 - 40.0 ng/mL.
Properties
PURITY:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
PHYSICAL STATE:
Lyophilized
STORAGE CONDITIONS:
The lyophilized HGF recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted HGF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing HGF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Additional Info
ALTERNATE NAMES:
SF, HGFB, HPTA, F-TCF, DFNB39, Hepatocyte growth factor, Hepatopoietin-A, SF
ACCESSION NO.:
NP_000592.3
PROTEIN GI NO.:
33859835
OFFICIAL SYMBOL:
HGF
GENE ID:
3082
Background and References
BACKGROUND:
HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear 697 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains (α-chain and β-chain) held by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 234 amino acid residues.
Specifications
