HES5 Antibody Cat. No.: 8055

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5.

The immunogen is located within the first 50 amino acids of HES5.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
SPECIFICITY: HES5 antibody is human, mouse and rat reactive. At least two isoforms of HES5 are known to exist; this antibody will detect both isoforms.
POSITIVE CONTROL:1) Cat. No. 1207 - Raji Cell Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 18 kDa

Observed: 17 kDa

psi-iconProperties
PURIFICATION:HES5 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:HES5 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:HES5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:HES5
ALTERNATE NAMES:Hairy and enhancer of split 5, hes family bHLH transcription factor 5, bHLHb38
ACCESSION NO.:NP_001010926
PROTEIN GI NO.:58219048
GENE ID:388585
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:HES5 is a member of the HES family of transcriptional repressors, which are the mammalian homologs of the Drosophila Hairy and Enhancer of Split and contain basic Helix-loop-helix (bHLH) and Orange domains (1). The HES5 gene is a downstream target of the Notch signaling pathway and its expression is downregulated during human cartilage differentiation (2). HES5 can directly repress transcription of the E3 ligase FBW7-beta, creating a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions (3).
REFERENCES:1) Katoh M and Katoh M. Identification and characterization of human HES2, HES3, and HES5 genes in silico. Int. J. Oncol. 2004; 25:529-34.
2) Karlsson C, Jonsson M, Asp J, et al. Notch and HES5 are regulated during human cartilage differentiation. Cell Tissue Res. 2007; 327:539-51.
3) Sancho R, Blake SM, Tendeng C, et al. Fbw7 repression by hes5 creates a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions. PLoS Biol. 2013; 11:e1001586.

ANTIBODIES FOR RESEARCH USE ONLY.

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