Datasheet

HDAC6 Antibody
CATALOG NUMBER: 7901

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:HDAC6 body can be used for detection of HDAC6 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1313 - Human Testis Tissue Lysate
 2) Cat. No. 11-701 - Human Testis Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 55 kDa

Observed: 32 kDa
SPECIFICITY:HDAC6 antibody is human and mouse reactive. Multiple isoforms of HDAC6 are known to exist. HDAC6 antibody is predicted to not cross-react with other members of the HDAC family.
IMMUNOGEN:HDAC6 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human HDAC6.

The immunogen is located within the last 50 amino acids of HDAC6.
HOST SPECIES:Rabbit

Properties

PURIFICATION:HDAC6 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:HDAC6 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:HDAC6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:HDAC6 Antibody: HD6, JM21, CPBHM, KIAA0901, Histone deacetylase 6, HD6
ACCESSION NO.:NP_006035
PROTEIN GI NO.:13128864
OFFICIAL SYMBOL:HDAC6
GENE ID:10013

Background

BACKGROUND:The histone deacetylase (HDAC) family contains multiple members which are divided into four classes. Class II of the HDAC family comprises six members, HDAC4, 5, 6, 7, 9 and 10, each of which appear to have tissue-specific roles (1). HDAC6 contains an internal duplication of two catalytic domains which appear to function independently of each other (2). HDAC6 has been shown to be part of the microtubule network and acts as a specific alpha-tubulin deacetylase, and has been suggested to be a potential therapeutic target in neurodegenerative disease (3).
REFERENCES: 1) de Ruijter AJ, van Gennip AH, Caron HN, et al. Histone deacetylases (HDACs): characterization of the classical HDAC family. Biochem. J. 2003; 370:737-49.
2) Grozinger CM, Hassig CA, and Schrieber SL. Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc. Natl. Acad. Sci. USA 1999; 96:4868-73.
3) Li G, Jiang H, Chang M, et al. HDAC6: a-tubulin decetylase: a potential therapeutic target in neurodegenerative disease. J. Neurol. Sci. 2011; 304:1-8.

For Research Use Only