Datasheet

HDAC2 Antibody
CATALOG NUMBER: 7899

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (94%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:HDAC2 antibody can be used for detection of HDAC2 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 54 kDa

Observed: 52 kDa
SPECIFICITY:HDAC2 antibody is human, mouse and rat reactive. At least two isoforms of HDAC2 are known to exist; this antibody will detect both isoforms. HDAC2 antibody is predicted to not cross-react with other members of the HDAC family.
IMMUNOGEN:HDAC2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human HDAC2.

The immunogen is located within the last 50 amino acids of HDAC2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:HDAC2 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:HDAC2 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:HDAC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:HDAC2 Antibody: HD2, RPD3, YAF1, Histone deacetylase 2, HD2
ACCESSION NO.:NP_001518
PROTEIN GI NO.:293336691
OFFICIAL SYMBOL:HDAC2
GENE ID:3066

Background

BACKGROUND:The histone deacetylase (HDAC) family contains multiple members which are divided into four classes. Class I of the HDAC family comprises four members, HDAC1, 2, 3, and 8, each of which contains a deacetylase domain and exhibits a different, individual substrate specificity and function in vivo (1). HDAC2 was first identified as as a mammalian homolog to the yeast transcriptional regulator RPD3 (2). HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor (2,3). Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events (4).
REFERENCES: 1) Taunton J, Hassig CA, and Schreiber SL. A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p. Science 1996; 272:408-11.
2) Yang WM, Inouye C, Zeng Y, et al. Transcriptional repression by YY1 is mediated by interaction with a mammalian homolog of the yeast global regulator RPD3. Proc. Natl. Acad. Sci. USA 1996; 93:12845-50.
3) Cress WD and Seto E. Histone deacetylases, transcriptional control, and cancer. J. Cell Phys. 2000; 184:1-16.
4) Kramer OH. HDAC2: a critical factor in health and disease. Trends Pharmacol. Sci. 2009; 30:647-55.

For Research Use Only