Datasheet

HDAC1 Antibody
CATALOG NUMBER: 7897

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:HDAC1 antibody can be used for detection of HDAC1 by Western blot at 1 - 2 μg/ml.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 53 kDa

Observed: 54 kDa
SPECIFICITY:HDAC1 antibody is human, mouse and rat reactive. HDAC1 antibody is predicted to not cross-react with other members of the HDAC family.
IMMUNOGEN:HDAC1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human HDAC1.

The immunogen is located within the last 50 amino acids of HDAC1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:HDAC1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:HDAC1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:HDAC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:HDAC1 Antibody: HD1, RPD3, GON-10, RPD3L1, Histone deacetylase 1, HD1
ACCESSION NO.:NP_004955
PROTEIN GI NO.:13128860
OFFICIAL SYMBOL:HDAC1
GENE ID:3065

Background

BACKGROUND:The histone deacetylase (HDAC) family contains multiple members which are divided into four classes. Class I of the HDAC family comprises four members, HDAC1, 2, 3, and 8, each of which contains a deacetylase domain and exhibits a different, individual substrate specificity and function in vivo (1). HDAC1 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (1,2). HDAC1 gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (3,4).
REFERENCES: 1) Taunton J, Hassig CA, and Schreiber SL. A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p. Science 1996; 272:408-11.
2) Cai RL, Yan-Neale Y, Cueto MA, et al. HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins. J. Biol. Chem. 2000; 275:27909-16.
3) Winter M, Moser MA, Meunier D, et al. Divergent roles of HDAC1 and HDAC2 in the regulation of epidermal development and tumorigenesis. EMBO J. 2013; 32:3176-91.
4) Turgeon N, Blais M, Gagné JM, et al. HDAC1 and HDAC2 restrain the intestinal inflammatory response by regulating intestinal epithelial cell differentiation. PLoS One 2013; 8:e73785.

For Research Use Only