| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Bovine: (94%) |
| IMMUNOGEN: | Hax1a antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human Hax1a. The immunogen is located within the first 50 amino acids of Hax1a. |
| TESTED APPLICATIONS: | ELISA, WB |
| APPLICATIONS: | Hax1a antibody can be used for detection of Hax1A by Western blot at 1 - 2 μg/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. |
| SPECIFICITY: | At least four isoforms of Hax1 are known to exist. This antibody is expected to recognize the longest isoform (Hax1a) as well as the shortest. |
| POSITIVE CONTROL: | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
Specifications| PURIFICATION: | Hax1a Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | Hax1a Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | Hax1a antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | HAX1 |
| ALTERNATE NAMES: | Hax1a Antibody: SCN3, HS1BP1, HCLSBP1, HCLS1-associated protein X-1, HS1-associating protein X-1, HAX-1 |
| ACCESSION NO.: | NP_006109 |
| PROTEIN GI NO.: | 13435356 |
| GENE ID: | 10456 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | Hax1a Antibody: The HS-1 associated protein X-1 (Hax1) was initially identified in a yeast two-hybrid assay on the basis of its ability to bind to the hemapoietic cell-specific protein 1 (HS-1). Hax1 possesses anti-apoptotic activity and is structurally related to Bcl-2 family members, including the presence of BH1- and BH2-like domains. It has recently been shown to interact with HIV viral protein R (Vpr), a protein required for viral pathogenesis of HIV and linked to T-cell apoptosis through activation of caspases 3 and 9. Other studies indicate that Hax1-mediated processing of HtrA2 (also known as Omi) by the mitochondrial protease PARL allows survival of lymphocytes and neurons when cytokines are limiting. |
| REFERENCES: | 1) Suzuki Y, Demoliere C, Kitamura D, et al. HAX-1, a novel intracellular protein, localized on mitochondria directly associates with HS1, a substrate of Src family tyrosine kinases. J. Immunol.1997; 158:2736-44. | 2) Sharp TV, Wang HW, Koumi A, et al. K15 protein of Kaposi’s sarcoma-associated herpesvirus is latently expressed and binds to HAX-1, a protein with antiapoptotic function. J. Virol.2002; 76:802-16. | 3) Yedavalli VS, Shih HM, Chiang YP, et al. Human immunodeficiency virus type 1 Vpr interacts with antiapoptotic mitochondrial protein HAX-1. J. Virol.2005; 79:13735-46. | 4) Chao J-R, Parganas E, Boyd K, et al. Hax1-mediated processing of HtrA2 by Parl allows survival of lymphocytes and neurons. Nature2008; 452:98-102. |
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