Hax1a Antibody Cat. No.: 4711

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (94%)
IMMUNOGEN: Hax1a antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human Hax1a.

The immunogen is located within the first 50 amino acids of Hax1a.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: Hax1a antibody can be used for detection of Hax1A by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
SPECIFICITY: At least four isoforms of Hax1 are known to exist. This antibody is expected to recognize the longest isoform (Hax1a) as well as the shortest.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate

psi-iconProperties
PURIFICATION:Hax1a Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Hax1a Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Hax1a antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:HAX1
ALTERNATE NAMES:Hax1a Antibody: SCN3, HS1BP1, HCLSBP1, HCLS1-associated protein X-1, HS1-associating protein X-1, HAX-1
ACCESSION NO.:NP_006109
PROTEIN GI NO.:13435356
GENE ID:10456
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Hax1a Antibody: The HS-1 associated protein X-1 (Hax1) was initially identified in a yeast two-hybrid assay on the basis of its ability to bind to the hemapoietic cell-specific protein 1 (HS-1). Hax1 possesses anti-apoptotic activity and is structurally related to Bcl-2 family members, including the presence of BH1- and BH2-like domains. It has recently been shown to interact with HIV viral protein R (Vpr), a protein required for viral pathogenesis of HIV and linked to T-cell apoptosis through activation of caspases 3 and 9. Other studies indicate that Hax1-mediated processing of HtrA2 (also known as Omi) by the mitochondrial protease PARL allows survival of lymphocytes and neurons when cytokines are limiting.
REFERENCES:1) Suzuki Y, Demoliere C, Kitamura D, et al. HAX-1, a novel intracellular protein, localized on mitochondria directly associates with HS1, a substrate of Src family tyrosine kinases. J. Immunol.1997; 158:2736-44.
2) Sharp TV, Wang HW, Koumi A, et al. K15 protein of Kaposi’s sarcoma-associated herpesvirus is latently expressed and binds to HAX-1, a protein with antiapoptotic function. J. Virol.2002; 76:802-16.
3) Yedavalli VS, Shih HM, Chiang YP, et al. Human immunodeficiency virus type 1 Vpr interacts with antiapoptotic mitochondrial protein HAX-1. J. Virol.2005; 79:13735-46.
4) Chao J-R, Parganas E, Boyd K, et al. Hax1-mediated processing of HtrA2 by Parl allows survival of lymphocytes and neurons. Nature2008; 452:98-102.

ANTIBODIES FOR RESEARCH USE ONLY.

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