Datasheet

GZF1 Antibody
CATALOG NUMBER: 6793

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (89%), Rat: (89%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:GZF1 antibody can be used for detection of GZF1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1301 - Human Heart Tissue Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
SPECIFICITY:GZF1 antibody is human specific. At least four isoforms of GZF1 are known to exist.
IMMUNOGEN:GZF1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human GZF1.

The immunogen is located within amino acids 600 - 650 of GZF1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:GZF1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:GZF1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GZF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:GZF1 Antibody: ZBTB23, ZNF336, ZBTB23, GDNF-inducible zinc finger protein 1, Zinc finger and BTB domain-containing protein 23
ACCESSION NO.:NP_071927
PROTEIN GI NO.:11968150
OFFICIAL SYMBOL:GZF1
GENE ID:64412

Background

BACKGROUND:GZF1 Antibody: The GDNF-inducible zinc finger protein 1 (GZF1) is a sequence-specific transcriptional repressor with a BTB/POZ domain and ten zinc finger motifs whose expression is required for renal branching morphogenesis during kidney development. GZF1 binds to the 5'regulatory region of the homeodomain protein HOXA10, suggesting that GZF1 may play a role in morphogenesis other than kidney development. Recent experiments have indicated that GZF1 associates with nucleolin and this association is mediated by the first four zinc finger motifs of GZF1. It is thought that Nucleolin modulates the subcellular localization of GZF1 as well as its transcriptional repressor activity.
REFERENCES: 1) Fukuda N, Ichihara M, Morinaga T, et al. Identification of a novel glial cell line-derived neurotrophic factor-inducible gene required for renal branching morphogenesis. J. Biol. Chem. 2003; 278:50386-92
2) Morinaga T, Enomoto A, Shimono Y, et al. GDNF-inducible zinc finger protein 1 is a sequence-specific transcriptional repressor that binds to the HOXA10 gene regulatory region. Nuc. Acids Res. 2005; 33:4191-201.
3) Dambara A, Morinaga T, Fukuda N, et al. Nucleolin modulates the subcellular localization of GDNF-inducible zinc finger protein 1 and its roles in transcription and cell proliferation. Exp. Cell Res. 2007; 313:3755-66.

For Research Use Only