GZF1 Antibody Cat. No.: 6793

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (89%), Rat: (89%)
IMMUNOGEN: GZF1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human GZF1.

The immunogen is located within amino acids 600 - 650 of GZF1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: GZF1 antibody can be used for detection of GZF1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: GZF1 antibody is human specific. At least four isoforms of GZF1 are known to exist.
POSITIVE CONTROL:1) Cat. No. 1301 - Human Heart Tissue Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide

psi-iconProperties
PURIFICATION:GZF1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:GZF1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GZF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:GZF1
ALTERNATE NAMES:GZF1 Antibody: ZBTB23, ZNF336, ZBTB23, GDNF-inducible zinc finger protein 1, Zinc finger and BTB domain-containing protein 23
ACCESSION NO.:NP_071927
PROTEIN GI NO.:11968150
GENE ID:64412
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:GZF1 Antibody: The GDNF-inducible zinc finger protein 1 (GZF1) is a sequence-specific transcriptional repressor with a BTB/POZ domain and ten zinc finger motifs whose expression is required for renal branching morphogenesis during kidney development. GZF1 binds to the 5'regulatory region of the homeodomain protein HOXA10, suggesting that GZF1 may play a role in morphogenesis other than kidney development. Recent experiments have indicated that GZF1 associates with nucleolin and this association is mediated by the first four zinc finger motifs of GZF1. It is thought that Nucleolin modulates the subcellular localization of GZF1 as well as its transcriptional repressor activity.
REFERENCES:1) Fukuda N, Ichihara M, Morinaga T, et al. Identification of a novel glial cell line-derived neurotrophic factor-inducible gene required for renal branching morphogenesis. J. Biol. Chem. 2003; 278:50386-92
2) Morinaga T, Enomoto A, Shimono Y, et al. GDNF-inducible zinc finger protein 1 is a sequence-specific transcriptional repressor that binds to the HOXA10 gene regulatory region. Nuc. Acids Res. 2005; 33:4191-201.
3) Dambara A, Morinaga T, Fukuda N, et al. Nucleolin modulates the subcellular localization of GDNF-inducible zinc finger protein 1 and its roles in transcription and cell proliferation. Exp. Cell Res. 2007; 313:3755-66.

ANTIBODIES FOR RESEARCH USE ONLY.

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