Datasheet

GSTP1 Antibody
CATALOG NUMBER: 4413

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (93%), Goat: (86%), Pig: (85%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:GSTP1 antibody can be used for the detection of ATG10 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 10-101 - Human Lung Tissue Slide
IMMUNOGEN:GSTP1 antibody was raised against a 14 amino acid synthetic peptide from near the center of human GSTP1.

The immunogen is located within amino acids 90 - 140 of GSTP1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:GSTP1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:GSTP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GSTP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:GSTP1 Antibody: PI, DFN7, GST3, GSTP, FAEES3, HEL-S-22, Glutathione S-transferase P, GST class-pi
ACCESSION NO.:NP_000843
PROTEIN GI NO.:4504183
OFFICIAL SYMBOL:GSTP1
GENE ID:2950

Background

BACKGROUND:GSTP1 Antibody: Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The glutathione S-transferase pi gene (GSTP1) is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism (i.e., the metabolism of environmental mutagens and carcinogens) and may play a role in susceptibility to cancer. More recent experiments have suggested that differential expression of GSTP1 also contributes to the sensitivity of xenobiotics in the substantia nigra and may influence the pathogenesis of reactive oxygen species-induced neurological disorders such as Parkinson's disease. CpG island hypermethylation of the GSTP1 promoter leading to the silencing of the GSTP1 gene has also been linked to cancer.
REFERENCES: 1) Pearson WR. Phylogenies of glutathione transferase families. Methods Enzymol.2005; 401:186-204.
2) Clapper ML. Genetic polymorphism and cancer risk. Curr. Oncol. Rep.2000; 2:251-6.
3) Smeyne M, Boyd J, Shepherd KR, etc. GSTpi expression mediates dopaminergic neuron sensitivity in experimental parkinsonism. Proc. Natl. Acad. Sci. USA2007; 104:1977-82.
4) Ellinger J, Bastian PJ, Jurgan T, et al. CpG island hypermethylation at multiple gene sites in diagnosis and prognosis of prostate cancer. Urology2008; 71:161-7.

For Research Use Only