GRIP1 Antibody Cat. No.: 7229

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Rabbit polyclonal GRIP1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human GRIP1.

The immunogen is located within the last 50 amino acids of GRIP1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: GRIP1 antibody can be used for detection of GRIP1 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 118 kDa

Observed: 115 kDa

psi-iconProperties
PURIFICATION:GRIP1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:GRIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GRIP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:GRIP1
ALTERNATE NAMES:GRIP1 Antibody: GRIP, Glutamate receptor-interacting protein 1, GRIP-1
ACCESSION NO.:NP_066973
PROTEIN GI NO.:223890252
GENE ID:23426
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:GRIP1 Antibody: Glutamate receptors play an important role in neural plasticity, development and degeneration. The glutamate receptor interacting proteins, GRIP1 and GRIP2, members of the PDZ domain-containing protein family, mediate the trafficking and membrane organization of a number of transmembrane proteins. GRIP1 and GRIP2 specifically bind to the AMPA receptor subunits, GluR 2/3 and are involved in the targeting of GluR 2/3 to the synapse. GRIP1 is expressed in early development before the expression of AMPA receptors, while GRIP2 expression parallels that of AMPA receptors during later developmental stages. GRIP1 and GRIP2 may be involved in the induction of cerebellar long-term depression (LTD).
REFERENCES:1) Nakanishi S. Molecular diversity of glutamate receptors and implications for brain function. Science 1992; 258:597-603.
2) Dong H, Zhang P, Song I, et al. Characterization of the glutamate receptor-interacting proteins GRIP1 and GRIP2. J. Neurosci. 1999; 19:6930-41.
3) Mao L, Takamiya K, Thomas G, et al. GRIP1 and 2 regulate activity-dependent AMPA receptor recycling via exocyst complex interactions. Proc. Natl. Acad. Sci. USA 2010; 107:19038-43.
4) Thomas GM, Hayashi T, Chiu SL, et al. Palmitoylation by DHHC5/8 targets GRIP1 to dendritic endosomes to regulate AMPA-R trafficking. Neuron 2012; 73:482-96.

ANTIBODIES FOR RESEARCH USE ONLY.

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