Datasheet

GRIP1 Antibody
CATALOG NUMBER: 7229

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:GRIP1 antibody can be used for detection of GRIP1 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 118 kDa

Observed: 115 kDa
IMMUNOGEN:Rabbit polyclonal GRIP1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human GRIP1.

The immunogen is located within the last 50 amino acids of GRIP1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:GRIP1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:GRIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GRIP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:GRIP1 Antibody: GRIP, Glutamate receptor-interacting protein 1, GRIP-1
ACCESSION NO.:NP_066973
PROTEIN GI NO.:223890252
OFFICIAL SYMBOL:GRIP1
GENE ID:23426

Background

BACKGROUND:GRIP1 Antibody: Glutamate receptors play an important role in neural plasticity, development and degeneration. The glutamate receptor interacting proteins, GRIP1 and GRIP2, members of the PDZ domain-containing protein family, mediate the trafficking and membrane organization of a number of transmembrane proteins. GRIP1 and GRIP2 specifically bind to the AMPA receptor subunits, GluR 2/3 and are involved in the targeting of GluR 2/3 to the synapse. GRIP1 is expressed in early development before the expression of AMPA receptors, while GRIP2 expression parallels that of AMPA receptors during later developmental stages. GRIP1 and GRIP2 may be involved in the induction of cerebellar long-term depression (LTD).
REFERENCES: 1) Nakanishi S. Molecular diversity of glutamate receptors and implications for brain function. Science 1992; 258:597-603.
2) Dong H, Zhang P, Song I, et al. Characterization of the glutamate receptor-interacting proteins GRIP1 and GRIP2. J. Neurosci. 1999; 19:6930-41.
3) Mao L, Takamiya K, Thomas G, et al. GRIP1 and 2 regulate activity-dependent AMPA receptor recycling via exocyst complex interactions. Proc. Natl. Acad. Sci. USA 2010; 107:19038-43.
4) Thomas GM, Hayashi T, Chiu SL, et al. Palmitoylation by DHHC5/8 targets GRIP1 to dendritic endosomes to regulate AMPA-R trafficking. Neuron 2012; 73:482-96.

For Research Use Only