Datasheet

Grik5 Antibody
CATALOG NUMBER: 4397

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Grik5 antibody can be used for detection of Grik5 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
SPECIFICITY:This Grik5 antibody does not cross-react with Grik4.
IMMUNOGEN:Grik5 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human Grik5.

The immunogen is located within the last 50 amino acids of Grik5.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Grik5 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Grik5 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Grik5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Grik5 Antibody: KA2, EAA2, GRIK2, GluK5, Glutamate receptor ionotropic, kainate 5, Excitatory amino acid receptor 2
ACCESSION NO.:NP_002079
PROTEIN GI NO.:29029597
OFFICIAL SYMBOL:GRIK5
GENE ID:2901

Background

BACKGROUND:Grik5 Antibody: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. Grik5, also known as kainate-preferring glutamate recptor subunit KA2, belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. Grik5 is highly homologous to the related ionotrophic glutamate receptor Grik4 (also known as KA1). Like Grik4, Grik5 does not form homomeric channels, but instead forms heteromers with Grik2. In Grik2- but not Grik1-null mice, Grik5 surface expression is greatly reduced in neurons, indicating that Grik2/Grik5 heteromers are required for exit from the endoplasmic reticulum to the cell surface.
REFERENCES: 1) Tanaka K. Functions of glutamate transports in the brain. Neurosci. Res.2000; 37:15-9.
2) Herb A, Burnashev N, Werner P, et al. The KA-2 subunit of excitatory amino acid receptors shows widespread expression in brain and forms ion channels with distantly related subunits. Neuron1992; 8:775-85.
3) Pinheiro P and Mulle C. Kainate receptors. Cell Tissue Res.2006; 326:457-82.
4) Nasu-Nishimura Y, Hurtado D, Braud S, et al. Tification of an endoplasmic reticulum-retention motif in an intracellular loop of the kainate receptor subunit KA2. J. Neurosci.2006; 26:7014-21.

For Research Use Only