Datasheet

Grik4 Antibody
CATALOG NUMBER: 4391

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Grik4 antibody can be used for detection of Grik4 by Western blot at 0.5 - 2 μg/mL. Despite its predicted molecular weight, Grik4 often migrates at a lower molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
IMMUNOGEN:Grik4 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the mouse Grik4.

The immunogen is located within the last 50 amino acids of Grik4.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Grik4 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Grik4 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Grik4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Grik4 Antibody: KA1, EAA1, GRIK, GluK4, Glutamate receptor ionotropic, kainate 4, Excitatory amino acid receptor 1
ACCESSION NO.:NP_780690
PROTEIN GI NO.:304766513
OFFICIAL SYMBOL:GRIK4
GENE ID:110637

Background

BACKGROUND:Grik4 Antibody: Grik4 codes for the KA1 subunit of kainate-type ionotropic glutamate receptors which are critical regulators of network activity that act by modifying neuronal excitability, directly and indirectly, through GABAergic interneurons. Five subunits can assemble to form kainate receptors (KARs): GluR5 (coded by Grik1), GluR6 (coded by Grik2), and GluR7 (coded by Grik3) are the low-affinity subunits, and KA1 and KA2 are the high-affinity subunits. In the adult brain, KARs are located pre- and postsynaptically on pyramidal cells and on interneurons. Kainate receptors on GABA-containing interneurons enhance GABA release and thereby downregulate glutamatergic signaling. KARs have been implicated in numerous psychiatric disorders. Case control studies show significant association of Grik4 with both schizophrenia and bipolar disorder.
REFERENCES: 1) Tanaka K. Functions of glutamate transporters in the brain. Neurosci. Res.2000; 37:15-9.
2) Pinheiro P and Mulle C. Kainate receptors. Cell Tissue Res.2006; 326:457-82.
3) Mayer ML. GRIK4 and the Kainate Receptor. Am. J. Psychiatry2007; 164:1148.
4) Pickard BS, Malloy MP, Christoforou A, et al. Cytogenetic and genetic evidence supports a role for the kainate-type glutamate receptor gene, GRIK4, in schizophrenia and bipolar disorder. Mol. Psychiatry2006; 11:847-57.

For Research Use Only