Datasheet

GRB10 Antibody
CATALOG NUMBER: 6733

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (100%)
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:GRB10 antibody can be used for detection of GRB10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1220 - SK-N-SH Cell Lysate
SPECIFICITY:At least three isoforms of GRB10 are known to exist; this antibody will detect all three isoforms.
IMMUNOGEN:GRB10 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GRB10.

The immunogen is located within amino acids 440 - 490 of GRB10.
HOST SPECIES:Rabbit

Properties

PURIFICATION:GRB10 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:GRB10 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GRB10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:GRB10 Antibody: RSS, IRBP, MEG1, GRB-IR, Grb-10, GRBIR, KIAA0207, Growth factor receptor-bound protein 10, GRB10 adapter protein
ACCESSION NO.:NP_005302
PROTEIN GI NO.:48762679
OFFICIAL SYMBOL:GRB10
GENE ID:2887

Background

BACKGROUND:GRB10 Antibody: GRB10 belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules and function in diverse cellular processes. GRB10 is a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of GRB10 inhibits tyrosine kinase activity and results in growth suppression. GRB10 can also interact with the L isoform of the proapoptotic protein Bim. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts.
REFERENCES: 1) Morrione A. Grb10 proteins in insulin-like growth factor and insulin receptor signaling (review). Int. J. Mol. Med. 2000; 5:151-4.
2) Liu F and Roth RA. Grb-IR: a SH2-domain-containing protein that binds to the insulin receptor and inhibits its function. Proc. Natl. Acad. Sci. USA 1995; 92:10287-91.
3) Wick KR, Werner ED, Langlais P, et al. Grb10 inhibits insulin-stimulated insulin receptor substrate (IRS)-phosphatidylinositol 3-kinase/Akt signaling pathway by disrupting the association of IRS-1/IRS-2 with the insulin receptor. J. Biol. Chem. 2003; 278:8460-7.
4) Hu ZQ, Zhang JY, Ji CN, et al. Grb10 interacts with Bim L and inhibits apoptosis. Mol. Biol. Rep. 2010; 37:3547-52.

For Research Use Only