Granzyme B Recombinant Protein Cat. No.: 40-125

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psi-iconSpecifications
SPECIES:Murine
SOURCE SPECIES:Baculovirus
SEQUENCE:IIGGHEVKPH SRPYMALLSI KDQQPEAICG GFLIREDFVL TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD VCYVAGWGRM APMGKYSNTL QEVELTVQKD RECESYFKNR YNKTNQICAG DPKTKRASFR GDSGGPLVCK KVAAGIVSYG YKDGSPPRAF TKVSSFLSWI KKTMKSS
TESTED APPLICATIONS:
BIOLOGICAL ACTIVITY: Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac - IEPD - pNA substrate at 25˚C, is greater than 750 nM/min per μg of enzyme.
psi-iconProperties
PURITY:Greater than 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin level is less than 0.1 ng per μg (1EU/μg).
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:The lyophilized Granzyme B recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Granzyme B is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Granzyme B recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
psi-iconAdditional Info
ALTERNATE NAMES:GZB, CCP1, Ctla1, Ctla-1, AI553453, CCP-1/C11, CTLA-1
ACCESSION NO.:NP_038570.1
PROTEIN GI NO.:7305123
OFFICIAL SYMBOL:Gzmb
GENE ID:14939
psi-iconBackground and References
BACKGROUND:Granzyme B is a cysteine protease found in the cytoplasmic granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B is required for the induction of target cell lysis, which occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms. Proteolytic cleavage of substrates by Granzyme B takes place primarily after aspartic acid residues. Recombinant murine Granzyme B is a glycosylated 227 amino acid protein, comprising the mature active portion of the murine Granzyme B precursor. The apparent molecular weight is 28.9 kDa by mass spectrometry.

FOR RESEARCH USE ONLY.

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