GPVI Antibody Cat. No.: 4769

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: GPVI antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of isoform 2 of the human GPVI.

The immunogen is located within the last 50 amino acids of GPVI.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: GPVI antibody can be used for detection of GPVI by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: At least three GPVI isoforms are known to exist; this antibody will detect the two longest isoforms. Because of its extensive glycosylation, GPVI often migrates at a higher than expected molecular weight in SDS-PAGE.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 68 kDa

Observed: 67 kDa

psi-iconProperties
PURIFICATION:GPVI Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:GPVI Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GPVI antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:GP6
ALTERNATE NAMES:GPVI Antibody: GPIV, GPVI, BDPLT11, Platelet glycoprotein VI, Glycoprotein 6
ACCESSION NO.:NP_057447
PROTEIN GI NO.:143770755
GENE ID:51206
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:GPVI Antibody: Glycoprotein VI (GP6) is a 58kD platelet membrane glycoprotein that plays a crucial role in the collagen-induced activation and aggregation of platelets. It is uniquely expressed by cells of the megakaryocytic/platelet lineage, and is a member of the immunoglobulin gene superfamily, closely related to Fc receptor gamma chain (FcRgamma) and natural killer receptors. Glycoprotein VI plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcRgamma, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2. GPVI deficiency can result in bleeding disorders. Further study should reveal the extent of GPVI involvement in thrombotic disease and allow the development of alternative anti-thrombotic compounds.
REFERENCES:1) Jarvis GE, Atkinson BT, Snell DC, et al. Distinct roles of GPVI and integrin alpha(2) beta(1) in platelet shape change and aggregation induced by different collagens. Br. J. Pharmacol. 2002; 137:107-17.
2) Inoue O, Suzuki-Inoue K, Dean WL, et al. Integrin alpha2beta1 mediates outside-in regulation of platelet spreading on collagen through activation of Src kinases and PLCgamma2. J. Cell Biol. 2003;160:769-80.
3) Clemetson JM, Polgar J, Magnenat E, et al. The platelet collagen receptor glycoprotein VI is a member of the immunoglobulin superfamily closely related to FcαR and the natural killer receptors. J Biol. Chem. 1999; 274:29019-24.
4) Jandrot-Perrus M, Busfield S, Lagrue AH, et al. Cloning, characterization, and functional studies of human and mouse glycoprotein VI: a platelet-specific collagen receptor from the immunoglobulin superfamily. Blood 2000; 96:1798-807.

ANTIBODIES FOR RESEARCH USE ONLY.

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