GPRASP1 Antibody Cat. No.: 7987

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
IMMUNOGEN: GPRASP1 antibody was raised against a 15 amino acid peptide near the amino terminus of human GPRASP1.

The immunogen is located within amino acids 260 - 310 of GPRASP1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: GPRASP1 antibody can be used for detection of GPRASP1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: GPRASP1 antibody is human and mouse reactive. This antibody is predicted to not cross-react with GPRASP2.
POSITIVE CONTROL:1) Cat. No. 1288 - A20 Cell Lysate
 2) Cat. No. 10-801 - Human Stomach Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 153 kDa

Observed: 150 kDa

psi-iconProperties
PURIFICATION:GPRASP1 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:GPRASP1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GPRASP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:GPRASP1
ALTERNATE NAMES:G-protein coupled receptor-associated sorting protein 1, GASP, GASP-1, GASP1
ACCESSION NO.:NP_001171656
PROTEIN GI NO.:296080706
GENE ID:9737
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The G protein-coupled receptor associated sorting protein 1 (GPRASP1) is a member of the GPRASP family and is thought to modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors (1,2). GPRASP1 interacts with receptors such as the D2 dopamine receptor (DRD2), delta opioid receptor (OPRD1), beta-2 adrenergic receptor (ADRB2), D4 dopamine receptor (DRD4) and cannabinoid 1 receptor (CNR1) and targets these proteins for degradation in lysosomes (2). Recently, it has been shown that increased GPRASP1 levels may act as a potential biomarker for breast cancer (3).
REFERENCES:1) Simonin F, Karcher P, Boeuf JJ, et al. Identification of a novel family of G protein-coupled receptor associated proteins. J. Neurochem. 2004; 89:766-75.
2) Moser E, Kargl J, Whistler JL, et al. G protein-coupled receptor-associated sorting protein 1 regulates the postendocytic sorting of seven-transmembrane-spanning G protein-coupled receptors. Pharmacology 2010; 86:22-9.
3) Tuszynski GP, Rothman VL, Zheng X, et al. G-protein coupled receptor-associated sorting protein 1 (GASP-1), a potential biomarker in breast cancer. Exp. Mol. Pathol. 2011; 91:608-13.
4) Yang Z, Qu X, and Yu CY. Features of the two gene pairs RD-SKI2W and DOM3Z-RP1 located between complement component genes factor B and C4 at the MHC class III region. Front. Biosci. 2001; 6:D927-35.

ANTIBODIES FOR RESEARCH USE ONLY.

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