GPR125 Antibody Cat. No.: 46-851

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY: Human
HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse
IMMUNOGEN: The immunogen for this antibody is: CTNKSASSFKNS
TESTED APPLICATIONS: ELISA, IHC, WB
APPLICATIONS: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 140kDa band observed in Human Ovary lysates (calculated MW of 146kDa according to NP_660333.2). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 33kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml. A customer reported successful results on Human Testis.
SPECIFICITY: #To_Delete#.
POSITIVE CONTROL:1) Cat. No. 1316 - Human Ovary Tissue Lysate

psi-iconProperties
PURIFICATION:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info
OFFICIAL SYMBOL:GPR125
ALTERNATE NAMES:GPR125 , G protein-coupled receptor 125 , PGR21, TEM5L, TEM5-like
ACCESSION NO.:NP_660333.2
PROTEIN GI NO.:59823631
GENE ID:166647
psi-iconBackground and References
REFERENCES:1) Seandel M, James D, Shmelkov SV, Falciatori I, Kim J, Chavala S, Scherr DS, Zhang F, Torres R, Gale NW, Yancopoulos GD, Murphy A, Valenzuela DM, Hobbs RM, Pandolfi PP, Rafii S. Generation of functional multipotent adult stem cells from GPR125+ germline progenitors. Nature. 2007 Sep 20;449(7160):346-50.

ANTIBODIES FOR RESEARCH USE ONLY.

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