GP120 Recombinant Protein Cat. No.: 96-366

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psi-iconSpecifications
SPECIES:HIV-1
SOURCE SPECIES:HEK293 cells
SEQUENCE:Thr 23 - Thr 652
FUSION TAG:His Tag
TESTED APPLICATIONS:WB
APPLICATIONS:This recombinant protein can be used for WB. For research use only.
BIOLOGICAL ACTIVITY: Measured by its binding ability in a functional ELISA. Immobilized HIV GP120 (HIV), His Tag at 5μg/mL (100 μL/well) can bind Human CD4, Fc Tag with a linear range of 4.9-78 ng/mL.
psi-iconProperties
PURITY:>97% as determined by SDS-PAGE.
PREDICTED MOLECULAR WEIGHT:53.8 kDa
PHYSICAL STATE:Lyophilized
BUFFER:PBS, pH7.4
STORAGE CONDITIONS:Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
psi-iconAdditional Info
ALTERNATE NAMES:GP120, GP120-CN54
ACCESSION NO.:Gene Num.: AX149771
psi-iconBackground and References
BACKGROUND:Human Immunodeficiency Virus (HIV) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to viruses found in chimpanzees and gorillas living in western Africa. HIV-2 is related to viruses found in sooty mangabeys. HIV-1 viruses may be further divided into groups. The HIV-1 group M viruses predominate and are responsible for the AIDS pandemic. Some of the HIV-1 group M subtypes are known to be more virulent or are resistant to different medications. HIV-2 viruses are thought to be less virulent and transmissible than HIV-1 M group viruses. Envelope glycoprotein GP120 (or gp120) is the name of the glycoprotein which forms the spikes sticking out of a HIV virus particle. gp120 is essential for virus entry into cells as it plays a vital role in seeking out specific cell surface receptors for entry. Three gp120s, bound as heterodimers to a transmembrane glycoprotein, gp41, are thought to combine in a trimer to form the envelope spike, which is involved in virus-cell attachment. One half of the molecular weight of gp120 is due to the carbohydrate side chains (the "glyco-" in "glycoprotein"). These are sugar residues which form something almost like a sugar "dome" over the gp120 spikes. This dome prevents gp120 from being recognised by the human immune response. As the HIV virus and the human CD4 cell come together, the gp120 binding site "snaps open" at the last minute.The glycoprotein gp120 is anchored to the viral membrane, or envelope, via non-covalent bonds with the transmembrane glycoprotein, gp41. It is involved in entry into cells by binding to CD4 receptors, particularly helper T-cells. Binding to CD4 is mainly electrostatic although there are van der Waals interactions and hydrogen bonds.
REFERENCES:1) Zhu P, et al., 2008, PLoS Pathog. 4 (11): e1000203.
2) Wood, N., et al., PLOS Pathogens 5: 1–16.
3) Kwong PD, et al., 1998, NATURE 393 (6686): 648-659.

FOR RESEARCH USE ONLY.

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