GOLPH2 Antibody Cat. No.: 5441

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: GOLPH2 antibody was raised against an 18 amino acid synthetic peptide from near the center of human GOLPH2.

The immunogen is located within amino acids 130 - 180 of GOLPH2.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: GOLPH2 antibody can be used for detection of GOLPH2 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 67 kDa

Observed: 70 kDa

psi-iconProperties
PURIFICATION:GOLPH2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:GOLPH2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GOLPH2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:GOLM1
ALTERNATE NAMES:GOLPH2 Antibody: GP73, HEL46, GOLPH2, C9orf155, PSEC0257, bA379P1.3, PSEC0242, UNQ686/PRO1326, Golgi membrane protein 1, Golgi membrane protein GP73
ACCESSION NO.:EAW62705
PROTEIN GI NO.:119583109
GENE ID:51280
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:GOLPH2 Antibody: GOLPH2, also known as GOLM1, is a Golgi phosphoprotein that has a short cytoplasmic N-terminal domain, a membrane spanning region, and a longer C-terminal domain. It was initially identified as a possible marker for Alzheimer's disease, although later studies have demonstrated that the GOLPH2 gene does not contribute to risk of this disease. GOLPH2 expression has been reported higher in prostate cancer tissues compared to normal prostate tissue, suggesting that GOLPH2 can be used as an additional positive marker for tissue-based diagnosis of prostate cancer. It has been suggested that GOLPH2 expression in hepatocellular carcinomas (HCCs) and serum may also serve as tumor markers for HCCs.
REFERENCES:1) Kladney RD, Bulla GA, Guo L, et al. GP73, a novel Golgi-localized protein upregulated by viral infection. Gene 2000; 249:53-65.
2) Li H, Wetten S, Li L, et al. Candidate single-nucleotide polymorphisms from a genomewide association study of Alzheimer disease. Arch. Neurol. 2008; 65:45-53.
3) Antunez C, Boada M, Lopez-Arrieta, et al. GOLPH2 gene markers are not associated with Alzheimer’s disease in a sample of the Spanish population. J. Alzheimers Dis. 2009; 18:751-4.
4) Kristiansen G, Fritzsche FR, Wassermann K, et al. GOLPH2 protein expression as a novel tissue biomarker for prostate cancer: implications for tissue-based diagnostics. Br. J. Cancer 2008; 99:939-48.

ANTIBODIES FOR RESEARCH USE ONLY.

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