GOLGIN97 Antibody Cat. No.: 8215

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: GOLGIN97 antibody was raised against a 17 amino acid peptide near the amino terminus of human GOLGIN97.

The immunogen is located within amino acids 30 - 80 of GOLGIN97.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: GOLGIN97 antibody can be used for detection of GOLGIN97 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
SPECIFICITY: GOLGIN97 antibody is human, mouse and rat reactive. At least two isoforms of GOLGIN97 are known to exist; this antibody will detect only the longer isoform. GOLGIN97 antibody is predicted to not cross-react with other GOLGIN family proteins.
POSITIVE CONTROL:1) Cat. No. 1319 - Human Stomach Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 84 kDa

Observed: 120 kDa

psi-iconProperties
PURIFICATION:GOLGIN97 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:GOLGIN97 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GOLGIN97 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:GOLGA1
ALTERNATE NAMES:Golgin subfamily A member 1, GOLGA1, GOLGIN A1, GOLGIN-97
ACCESSION NO.:NP_002068
PROTEIN GI NO.:4504063
GENE ID:2800
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:GOLGIN97 was initially isolated as a Golgi complex autoantigen associated with Sjogren’s syndrome, a chronic autoimmune disease in which the body's white blood cells destroy the exocrine glands, specifically the salivary and lacrimal glands (1,2). GOLGIN97 plays a role as a tethering molecule in endosome to the trans-Golgi network (TGN) retrograde traffic (3). FIP1/RCP binding to GOLGIN97 is required for tethering and fusion of recycling endosome retrograde transport vesicles to the TGN (4).
REFERENCES:1) Griffith KJ, Chan EK, Lung CC, et al. Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjogren’s syndrome. Arthritis Rheum. 1997; 40:1693-702.
2) Borchers AT, Naguwa SM, Keen CL, et al. Immunopathogenesis of Sjogren's syndrome. Clin. Rev. Allergy Immunol. 2003; 25:89-104.
3) Lu L, Tai G, and Hong W. Autoantigen Golgin-97, and effector of ARL1 GTPase, participates in traffic from the endosome to the trans-Golgi network. Mol. Biol. Cell 2004; 15:4426-43.
4) Jing J, Junutula JR, Wu C, et al. FIP1/RCP binding to Golgin-97 regulates retrograde transport from recycling endosomes to the trans-Golgi network. Mol. Biol. Cell 2010; 21:3041-53.

ANTIBODIES FOR RESEARCH USE ONLY.

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