Datasheet

GOLGA5 Antibody
CATALOG NUMBER: 7387

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:GOLGA5 antibody can be used for detection of GOLGA5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1313 - Human Testis Tissue Lysate
 2) Cat. No. 11-701 - Human Testis Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 64, 80 kDa

Observed: 65, 78 kDa
SPECIFICITY:GOLGA5 antibody is human, mouse and rat reactive. Two isoforms of GOLGA5 exist due to alternative splicing events.
IMMUNOGEN:GOLGA5 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human GOLGA5.

The immunogen is located within amino acids 540 - 590 of GOLGA5.
HOST SPECIES:Rabbit

Properties

PURIFICATION:GOLGA5 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:GOLGA5 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GOLGA5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:GOLGA5 Antibody: RFG5, GOLIM5, ret-II, RETII, RFG5, PIG31, Golgin subfamily A member 5, Cell proliferation-inducing gene 31 protein
ACCESSION NO.:NP_005104
PROTEIN GI NO.:30260188
OFFICIAL SYMBOL:GOLGA5
GENE ID:9950

Background

BACKGROUND:GOLGA5 Antibody: GOLGA5 (Golgi autoantigen, subfamily A, member 5), also known as GOLGA84, RFG5 (RET-fused gene 5 protein) or GOLIM5, is a ubiquitously expressed single-pass type II coiled-coil membrane protein that is involved in maintaining Golgi structure (1). It is localized to the Golgi apparatus and predominantly found on membranes at the cis side of the Golgi stack (1,2). As a mitotic phosphoprotein, GOLGA5 binds to active Rab 1 and associates with CASP (an isoform of CDP) in a golgin-tethering complex that is believed to play a role in intra-Golgi retrograde transport (3,4). Defects in GOLGA5 are a cause of thyroid papillary carcinoma (TPC) (5).
REFERENCES: 1) Bascom RA, Srinivasan S, Nussbaum RL. Identification and characterization of golgin 84, a novel Golgi integral membrane protein with a cytoplasmic coiled-coil domain. J. Biol. Chem. 1999; 274:2953-62.
2) Diao A, Rahman D, Pappin DJ, et al. 2003. The coiled-coil membrane protein golgin 84 is a novel Rab effector required for Golgi ribbon formation. J. Cell Biol. 2003; 160:201-12.
3) Malsam J, Satoh A, Pelletier L, et al. Golgin tethers define subpopulations of COPI vesicles. Science 2005; 307:1095-8.
4) Sohda M, Misumi Y, Yamamoto A, et al. Interaction of Golgin-84 with the COG complex mediates the intra-Golgi retrograde transport. Traffic 2010;11:1552-66.

For Research Use Only