Goat Anti-Mouse IgG (H&L) Antibody (HRP) Cat. No.: XR-9720

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY:Mouse
IMMUNOGEN:Mouse IgG - whole molecule
CONJUGATE:HRP
TARGET SPECIES:Reacts with the heavy chain of mouse IgG.
TESTED APPLICATIONS:ELISA, IHC, WB
APPLICATIONS:Peroxidase Conjugated Affinity Purified anti-Mouse IgG is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:50,000 - 1:200,000, Western Blot 1:4,000 - 1:20,000, Immunohistochemistry 1:1,000 - 1:8,000. (Optimal dilution should be determined by user).
SPECIFICITY:Minimum Cross reactivity to Human Serum Proteins
psi-iconProperties
PURIFICATION:Peroxidase Conjugated Affinity Purified anti-Mouse IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins.
ISOTYPE:IgG
CONJUGATE:HRP
PHYSICAL STATE:Lyophilized
BUFFER:0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/mL Bovine Serum Albumin (BSA); 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
STORAGE CONDITIONS:Peroxidase Conjugated Affinity Purified anti-Mouse IgG can be stored vial at 4 ˚C prior to restoration. For extended storage aliquot contents and freeze at -20 ˚C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 ˚C as an undiluted liquid. Dilute only prior to immediate use.
psi-iconBackground and References
REFERENCES:1) Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.

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