Datasheet

GNPDA2 Antibody
CATALOG NUMBER: 5061

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:GNPDA2 antibody can be used for detection of GNPDA2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1305 - Human Kidney Tissue Lysate
 2) Cat. No. 17-010 - HEK293 Cell Slide
SPECIFICITY:GNPDA2 antibody is predicted to not cross-react with GNPDA1.
IMMUNOGEN:GNPDA2 antibody was raised against an 8 amino acid synthetic peptide near the carboxy terminus of human GNPDA2.

The immunogen is located within the last 50 amino acids of GNPDA2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:GNPDA2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:GNPDA2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GNPDA2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:GNPDA2 Antibody: GNP2, SB52, GNP2, Glucosamine-6-phosphate isomerase 2, Glucosamine-6-phosphate deaminase 2, GNPDA 2
ACCESSION NO.:NP_612208
PROTEIN GI NO.:19923881
OFFICIAL SYMBOL:GNPDA2
GENE ID:132789

Background

BACKGROUND:GNPDA2 Antibody: Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA2 is the second mammalian glucosamine-6-phosphate deaminase discovered, and is closer in structure and activity to the E. coli enzyme than GNPDA1. GNPDA1 possesses greater affinity for ammonium than either GNPDA2 or the E. coli enzyme suggesting that the forward reaction of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium is catalyzed at a slower rate than GNPDA2.
REFERENCES: 1) Wolosker H, Kline D, Bian Y, et al. Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity. FASEB J.1998; 12:91-9.
2) Zhang J, Zhang W, Zou D, et al. Cloning and functional characterization of GNPI, a novel human homolog of glucosamine-6-phosphate isomerase/oscillin. J. Cell Biochem.2003; 88:932-40.
3) Arreola R, Valderrama B, Morante ML, et al. Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic study. FEBS Lett.2003; 551:63-70.

For Research Use Only