Datasheet

GNPDA1 Antibody
CATALOG NUMBER: 5049

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (94%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:GNPDA1 antibody can be used for detection of GNPDA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1405 - Mouse Kidney Tissue Lysate
 2) Cat. No. 17-010 - HEK293 Cell Slide
SPECIFICITY:GNPDA1 is predicted to not cross-react with GNPDA2.
IMMUNOGEN:GNPDA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human GNPDA1.

The immunogen is located within the last 50 amino acids of GNPDA1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:GNPDA1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:GNPDA1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GNPDA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:GNPDA1 Antibody: GPI, HLN, GNP1, GNPI, GNPDA, KIAA0060, Glucosamine-6-phosphate isomerase 1, Glucosamine-6-phosphate deaminase 1, GNPDA 1
ACCESSION NO.:AAC05123
PROTEIN GI NO.:2935438
OFFICIAL SYMBOL:GNPDA1
GENE ID:10007

Background

BACKGROUND:GNPDA1 Antibody: Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA1 is one of two mammalian glucosamine-6-phosphate deaminases that are thought to have arisen though gene duplication, with the GNPDA2 protein closer in structure and activity to the E. coli enzyme. GNPDA1 possesses greater affinity for ammonium than either GNPDA2 or the E. coli enzyme suggesting that the forward reaction of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium is catalyzed at a slower rate than GNPDA2.
REFERENCES: 1) Wolosker H, Kline D, Bian Y, et al. Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity. FASEB J.1998; 12:91-9.
2) Zhang J, Zhang W, Zou D, et al. Cloning and functional characterization of GNPI, a novel human homolog of glucosamine-6-phosphate isomerase/oscillin. J. Cell Biochem.2003; 88:932-40.
3) Arreola R, Valderrama B, Morante ML, et al. Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic study. FEBS Lett.2003; 551:63-70.

For Research Use Only