Datasheet

GLS2 Antibody
CATALOG NUMBER: 6217

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:IF, IHC-P, WB
APPLICATIONS:GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1464 - Rat Liver Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 36, 55, 62, 66 kDa

Observed: 55, 62 kDa
SPECIFICITY:Multiple isoforms of GLS2 are known to exist.
IMMUNOGEN:GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2.

The immunogen is located within amino acids 300 - 350 of GLS2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:GLS2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:GLS2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GLS2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:GLS2 Antibody: GA, GLS, LGA, hLGA, GA, Glutaminase liver isoform, mitochondrial, L-glutaminase
ACCESSION NO.:NP_037399
PROTEIN GI NO.:20336214
OFFICIAL SYMBOL:GLS2
GENE ID:27165

Background

BACKGROUND:GLS2 Antibody: Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53's role in antioxidant defense in addition to its role in energy metabolism.
REFERENCES: 1) Chung-Bok MI, Vincent N, Jhala U, et al. Rat hepatic glutaminase: identification of the full coding sequence and characterization of a functional promoter. Biochem. J. 1997; 324:193-200.
2) Gomez-Fabre PM, Aledo JC, del Castillo-Olivares A, et al. Molecular cloning, sequencing and expression studies of the human breast cancer cell glutaminase. Biochem. J. 2000; 345:365-75.
3) Hu W, Zhang C, Wu R, et al. Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function. Proc. Natl. Acad. Sci. USA 2010; 107:7455-60.

For Research Use Only