Datasheet

Gle1 Antibody
CATALOG NUMBER: 4963

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Gle1 antibody can be used for detection of Gle1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate
IMMUNOGEN:Gle1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human Gle1.

The immunogen is located within amino acids 540 - 590 of Gle1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Gle1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Gle1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Gle1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Gle1 Antibody: LCCS, GLE1L, LCCS1, hGLE1, Nucleoporin GLE1, GLE1-like protein
ACCESSION NO.:NP_001003722
PROTEIN GI NO.:51317384
OFFICIAL SYMBOL:GLE1
GENE ID:2733

Background

BACKGROUND:Gle1 Antibody: The proper expression of gene products in eukaryotic cells relies on efficient transport of mRNA molecules out of the nucleus. Gle1 is an essential mRNA export factor in both human and yeast cells. It associates with the nuclear pore complex (NPC) through hCG1 and NUP155 in mammalian cells and in conjunction with inositol hexakisphosphate (IP6), stimulates Dbp5, a member of the DEAD-box helicase family, triggering mRNP remodeling and facilitating RNA export from the nucleus. Recent evidence suggests that mutations in Gle1 causing defects in mRNA export can result in human disease. At least three isoforms of Gle1 are known to exist.
REFERENCES: 1) Murphy R and Wente SR. An RNA-export mediator with an essential nuclear export signal. Nature1996; 383:357-60.
2) Watkins JL, Murphy R, Emtage JL, et al. The human homologue of Saccharomyces cerevisiae Gle1p is required for poly(A)+ RNA export. Proc. Natl. Acad. Sci. USA1998; 95:6779-84.
3) Kendirgi F, Rexer DJ, Alcazar-Roman AR, et al. Interaction between the shuttling mRNA export factor Gle1 and the nucleoporin hCG1: A conserved mechanism in the export of Hsp70 mRNA. Mol. Biol. Cell2005; 16:4304-15.
4) Rayala HJ, Kendirgi F, Barry DM, et al. The mRNA export factor human Gle1 interacts with the nuclear pore complex protein Nup155. Mol. Cell. Proteomics2004; 3:145-55.

For Research Use Only