Datasheet

GJB2 Antibody
CATALOG NUMBER: 8031

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (94%), Mouse: (94%), Sheep: (88%), Bovine: (88%), Chicken: (82%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:GJB2 antibody can be used for detection of GJB2 by Western blot at 1 - 2 μg/ml.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1320 - Human Colon Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 25 kDa

Observed: 26 kDa
SPECIFICITY:GJB2 antibody is human specific.
IMMUNOGEN:GJB2 antibody was raised against a 16 amino acid peptide near the center of human GJB2.

The immunogen is located within amino acids 80 - 130 of GJB2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:GJB2 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:GJB2 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GJB2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Gap junction beta-2 protein, connexin 26, CX26, DFNA3, DFNA3A, DFNB1, DFNB1A, HID, KID, NSRD1, PPK
ACCESSION NO.:NP_003995
PROTEIN GI NO.:42558283
OFFICIAL SYMBOL:GJB2
GENE ID:2706

Background

BACKGROUND:The Gap junction beta-2 protein (GJB2), also known as Connexin 26, is member of the gap junction protein family which form structures that were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells (1). Mutations in the GJB2 gene are thought to be responsible for as much as 35-45% of congenital sensorineural hearing loss in some populations (2). Other mutations in this gene have also been linked to a wide array of skin diseases (3).
REFERENCES: 1) Zhou JZ and Jiang JX. Gap junctions and hemichannel-independent actions of connexins on cell and tissue functions – An update. FEBS Lett. 2014; 588:1186-92.
2) Petit C, Levilliers J, and Hardelin JP. Molecular genetics of hearing loss. Annu. Rev. Genet. 2001; 35:589-646.
3) Gerido DA and White TW. Connexin disorders of the ear, skin, and lens. Biochim. Biophys. Acta. 2004; 1662:159-70.

For Research Use Only