| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Bovine: (93%) |
| IMMUNOGEN: | GFR alpha 2 antibody was raised against a 15 amino acid peptide near the center of human GFR alpha 2. The immunogen is located within amino acids 360 - 410 of GFR alpha 2. |
| TESTED APPLICATIONS: | ELISA, ICC, WB |
| APPLICATIONS: | GFR alpha 2 antibody can be used for detection of GFR alpha 2 by Western blot at 1 μg/mL. A 52 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. |
| POSITIVE CONTROL: | 1) Cat. No. 1201 - HeLa Cell Lysate |
| 2) Cat. No. 17-001 - HeLa Cell Slide | |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 51 kDa Observed: 52 kDa |
Specifications| PURIFICATION: | GFR alpha 2 Antibody is Ion exchange chromatography purified. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | GFR alpha 2 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | batch dependent |
| STORAGE CONDITIONS: | GFR alpha 2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | GFRA2 |
| ALTERNATE NAMES: | GFR alpha 2 Antibody: NTNRA, RETL2, TRNR2, GDNFRB, NRTNR-ALPHA, GDNF family receptor alpha-2, GDNF receptor beta, GDNF receptor alpha-2 |
| ACCESSION NO.: | AAC52036 |
| PROTEIN GI NO.: | 2145080 |
| GENE ID: | 2675 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | GFR alpha 2 Antibody: Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN) play key roles in the control of vertebrate neuron survival and differentiation. Physiological responses to NTN require the presence of a novel glycosylphosphadidylinositol linked protein NTNRalpha, which is a cell surface receptor for NTN. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned recently. NTNRalpha was also termed GDNFRβ, Ret ligand 2 (RETL2) or TGFbeta-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRα-2 recently. GFRα-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFRα-2, and the Ret PTK form a complex to transduce NTN signal and to mediate NTN function. |
| REFERENCES: | 1) Kotzbauer PT, Lampe PA, Heuckeroth RO, et al. Neurturin, a relative glial-cell-line-derived neurotrophic factor. Nature 1996; 384:467-70. | 2) Klein RD, Sherman D, Ho WH, et al. A GPI-linked protein that interacts with Ret to form a candidate neurturin receptor. Nature 1997; 387:717-21. | 3) Buj-Bello A, Adu J, Pinon LG, et al. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997; 387:721-4. | 4) Baloh RH, Tansey MG, Golden JP, et al. TrnR2, a novel receptor that mediates neurturin and GDNF signaling through Ret. Neuron 1997; 18:793-802. |
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