| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| IMMUNOGEN: | GFR alpha 1 antibody was raised against a 14 amino acid peptide near the center of human GFR alpha 1. The immunogen is located within amino acids 360 - 410 of GFR alpha 1. |
| TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
| APPLICATIONS: | GFR alpha 1 antibody can be used for detection of GFR alpha 1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
| SPECIFICITY: | GFR alpha 1 antibody is predicted to not cross-react with other members of the GFR alpha family of proteins. |
| POSITIVE CONTROL: | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
| 2) Cat. No. 1304 - Human Liver Tissue Lysate | |
| 3) Cat. No. 1305 - Human Kidney Tissue Lysate | |
| 4) Cat. No. 1306 - Human Spleen Tissue Lysate | |
| 5) Cat. No. 10-301 - Human Brain Tissue Slide | |
| PREDICTED MOLECULAR WEIGHT: | 51 kDa |
Specifications| PURIFICATION: | GFR alpha 1 Antibody is Antibody is DEAE purified. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | GFR alpha 1 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | GFR alpha 1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | GFRA1 |
| ALTERNATE NAMES: | GFR alpha 1 Antibody: GDNFR, RET1L, RETL1, TRNR1, GDNFRA, GFR-ALPHA-1, GDNF family receptor alpha-1, RET ligand 1, GDNF receptor alpha-1 |
| ACCESSION NO.: | P56159 |
| PROTEIN GI NO.: | 20141405 |
| GENE ID: | 2674 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | GFR alpha 1 Antibody: Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRalpha, which is a cell surface receptor for GDNF. The cDNAs encoding GDNFRalpha from human, rat, chicken and mouse have been cloned recently. GDNFRalpha was also termed Ret ligand 1 (RETL1) or TGF-beta-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFRα-1 recently. GFRα-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRα and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function. |
| REFERENCES: | 1) Jing S, Wen D, Yu Y, et al. GDNF-induced activation of the Ret protein tyrosine kinase is mediated by GDNFR-a, a novel receptor for GDNF. Cell 1996; 85:1113-24. | 2) Treanor JJS, Goodman L, Sauvage FD, et al. Characterization of a multicomponent receptor for GDNF. Nature 1996;82:80-83. | 3) Sanicola M, Hession C, Worley D, et al. Glial cell line-derived neurotrophic factor-dependent RET activation can be mediated by two different cell-surface accessory proteins. Proc. Natl. Acad. Sci. USA 1997; 94:6238-43. | 4) Buj-Bello A, Adu J, Pinon LG, et al. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997; 387:721-4 |
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