GFP Antibody [4B10B2] Cat. No.: 32-160

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: GFP
IMMUNOGEN: Ni-NTA purified full-length recombinant GFP-His expressed in E. Coli strain BL21 (DE3).
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

psi-iconProperties
CLONALITY:Monoclonal
ISOTYPE:IgG2a
CONJUGATE:Unconjugated
BUFFER:Purified antibody in PBS containing 0.03% sodium azide.
STORAGE CONDITIONS:GFP monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:gfp
ALTERNATE NAMES:Green fluorescent protein
ACCESSION NO.:YP_002302326.1
PROTEIN GI NO.:211909965
GENE ID:7011691
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
REFERENCES:1) Dittman JS. Kaplan JM. Proc Natl Acad Sci U S A. 2006, Jul 25,103(30):11399-404. Epub 2006 Jul 14.
2) Cavalieri V. Bernardo MD. Spinelli G. Gene Expr Patterns. 2007, Jan,7(1-2):124-30. Epub 2006 Jun 14.
3) Arasada R. Son H. Ramalingam N. et al. Eur J Cell Biol. 2006, Sep,85(9-10):1059-68. Epub 2006 Jul 13.

ANTIBODIES FOR RESEARCH USE ONLY.

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