GEF5 Antibody Cat. No.: 45-664

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY: Human
IMMUNOGEN: The immunogen for this antibody is: C-DKEIDQNSQQEE
TESTED APPLICATIONS: ELISA, IHC, WB
APPLICATIONS: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 170kDa band observed in Human HeLa lysates (calculated MW of 177kDa according to NP_005426.2). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml.
SPECIFICITY: #To_Delete#.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate

psi-iconProperties
PURIFICATION:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info
OFFICIAL SYMBOL:ARHGEF5
ALTERNATE NAMES:GEF5, DKFZp686N1969, ARHGEF5, Rho guanine nucleotide exchange factor (GEF) 5, P60, TIM, TIM1, guanine nucleotide regulatory protein TIM, oncogene TIM rho guanine nucleotide exchange factor 5, transforming immortalized mammary oncogene
ACCESSION NO.:NP_005426.2
PROTEIN GI NO.:50959191
GENE ID:7984
psi-iconBackground and References
REFERENCES:1) Debily MA, Camarca A, Ciullo M, Mayer C, El Marhomy S, Ba I, Jalil A, Anzisi A, Guardiola J, Piatier-Tonneau D. Expression and molecular characterization of alternative transcripts of the ARHGEF5/TIM oncogene specific for human breast cancer. Hum Mol Genet. 2004 Feb 1;13(3):323-34. Epub 2003 Dec 8.

ANTIBODIES FOR RESEARCH USE ONLY.

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