The ED50 was determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3/E1 cells. The ED50 for this effect is 8-10 ng/ml.
Properties
PURITY:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
PHYSICAL STATE:
Lyophilized
STORAGE CONDITIONS:
The lyophilized GDF-11 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted GDF-11 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing GDF-11 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Additional Info
ALTERNATE NAMES:
BMP11, BMP-11, BMP11, Bone morphogenetic protein 11, GDF-11
ACCESSION NO.:
NP_005802.1
PROTEIN GI NO.:
5031613
OFFICIAL SYMBOL:
GDF11
GENE ID:
10220
Background and References
BACKGROUND:
GDF-11 is a myostatin-homologous protein that acts as an inhibitor of nerve tissue growth. GDF-11 has been shown to suppress neurogenesis through a myostatin-like pathway, which involves arrest of progenitor cell-cycle in the G1 phase. Similarities between myostatin and GDF-11, which are 90% identical in their amino acid sequence, suggests that the regulatory mechanisms responsible for maintaining proper tissue size during neural and muscular development might be the same. Recombinant human GDF-11 is a 25.0 kDa disulfide-linked homodimer containing two 109 amino acid polypeptide chains. It is highly homologous to myostatin/GDF-8 sharing 90% amino-acid sequence identity.
Specifications
