Datasheet

GATA3 Antibody
CATALOG NUMBER: 5599

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (100%), Pig: (72%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:GATA3 antibody can be used for detection of GATA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1204 - K562 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 49 kDa

Observed: 49 kDa
IMMUNOGEN:GATA3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GATA3.

The immunogen is located within amino acids 340 - 390 of GATA3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:GATA3 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:GATA3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GATA3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:GATA3 Antibody: HDR, HDRS, Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3
ACCESSION NO.:NP_001002295
PROTEIN GI NO.:50541959
OFFICIAL SYMBOL:GATA3
GENE ID:2625

Background

BACKGROUND:GATA3 Antibody: GATA3 is a zinc finger transcription factor that was first recognized as a possible determinant of T cell development. Later studies demonstrated that it is expressed in trophoblast giant cells and regulates trophoblast-specific genes during development. Specifically, GATA3 is selectively expressed in the trophectoderm of the peri-implantation embryo and regulates CDX2, which is required for the transcriptional repression of the POU5F1/Oct4 and NANOG genes and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Decreased or null-expression expression of GATA3 has also been suggested to play a major role in the development and progression of luminal breast cancer.
REFERENCES: 1) Ko LJ, Yamamoto M, Leonard MW, et al. Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer. Mol. Cell. Biol. 1991; 11:2778-84.
2) Ma GT, Roth ME, Groskopf JC, et al. GATA-2 and GATA-3 regulate trophoblast-specific gene expression in vivo. Dev. 1997; 124:907-14.
3) Home P, Ray S, Dutta D, et al. GATA3 is selectively expressed in the trophectoderm of peri-implantation embryo and directly regulates Cdx2 gene expression. J. Biol. Chem. 2009; 284:28729-37.
4) Strumpf D, Mao CA, Yamanaka Y, et al. CDX2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst. Dev. 2005; 132:2093-102.

For Research Use Only