Datasheet

GAS Antibody
CATALOG NUMBER: 5437

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (93%), Rat: (93%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:GAS antibody can be used for detection of GAS by Western blot at 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1287 - EL4 Cell Lysate
IMMUNOGEN:GAS antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human GAS.

The immunogen is located within the last 50 amino acids of GAS.
HOST SPECIES:Rabbit

Properties

PURIFICATION:GAS Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:GAS Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GAS antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:GAS Antibody: GAS, PA1, C16orf53, PAXIP1-associated glutamate-rich protein 1, PAXIP1-associated protein 1
ACCESSION NO.:NP_078792
PROTEIN GI NO.:13375654
OFFICIAL SYMBOL:PAGR1
GENE ID:79447

Background

BACKGROUND:GAS Antibody: Steroid receptor co-activators (SRCs) were initially described as nuclear receptor transcription co-activators, but they have recently been determined to co-regulate transcription initiated by other transcription factors. GAS is a recently identified glutamate-rich protein that interacts with SRC1, but not GRIP1 or AIB1, the other two members of the SRC family. GAS can also interact with the alpha subunit of the estrogen receptor (ERalpha), but not other receptors such as the retinoic acid receptor a, suggesting the interaction between GAS and ERalpha is relatively specific. Depletion of GAS by RNA interference in MCF7 cells led to a decrease in the mRNA and protein levels of ER target genes such as pS2, c-Myc and cyclin D1, indicating the role of GAS in the regulation of ER target genes. GAS has also been found to associate with an SET1-like methyltransferase complex specific for H3K4 methylation, suggesting that GAS has multiple roles in transcriptional regulation.
REFERENCES: 1) Xu J, Wu RC, and O’Malley BW. Normal and cancer-related functions of the p160 steroid receptor co-activator (SCR) family. Nat. Rev. Cancer2009; 9:615-30.
2) Liang J, Zhang H, Zhang Y, et al. GAS, a new glutamate-rich protein, interacts differentially with SRCs and is involved in oestrogen receptor function. EMBO Rep.2009; 10:51-7.
3) PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex. J. Biol. Chem.2007; 13:20395-406.

For Research Use Only