GAPDH Antibody (HRP) Cat. No.: 3781-HRP

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Chicken, Human, Mouse, Rabbit, Rat
IMMUNOGEN: HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: HRP-GAPDH antibody can be used for detection of GAPDH by Western blot at 0.5 - 1 μ/ml.

Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
SPECIFICITY: HRP-GAPDH antibody is human, mouse, rat, rabbit, and chicken reactive.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 37 kDa

Observed: 36 kDa

psi-iconProperties
PURIFICATION:HRP-GAPDH antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:HRP
PHYSICAL STATE:Liquid
BUFFER:HRP-GAPDH antibody is supplied in PBS containing 1% BSA and 0.02% thimerosol.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:HRP-GAPDH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:GAPDH
ALTERNATE NAMES:HRP-GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
ACCESSION NO.:NP_002037
PROTEIN GI NO.:7669492
GENE ID:2597
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
REFERENCES:1) Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.
2) Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.

ANTIBODIES FOR RESEARCH USE ONLY.

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