Datasheet

GAPDH [12D3H9] Antibody
CATALOG NUMBER: PM-7571

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (100%), Guinea pig: (100%), Sheep: (93%), Bovine: (93%), Chicken: (93%), Rabbit: (93%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μg/ml.

Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 37 kDa

Observed: 36 kDa
SPECIFICITY:GAPDH antibody is human, mouse,and rat reactive.
IMMUNOGEN:GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.

The immunogen is located within amino acids 230 - 280 of GAPDH.
HOST SPECIES:Mouse

Properties

PURIFICATION:GAPDH antibody is Protein A purified.
PHYSICAL STATE:Liquid
BUFFER:GAPDH antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GAPDH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Monoclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
ACCESSION NO.:NP_002037
PROTEIN GI NO.:7669492
OFFICIAL SYMBOL:GAPDH
GENE ID:2597

Background

BACKGROUND:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
REFERENCES: 1) Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.
2) Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.

For Research Use Only