Galectin-1 Antibody (biotin) Cat. No.: 38-141

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGalectin-1. Human Galectin-1 specific antibody was purified by affinity chromatography and then biotinylated.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: ELISA:

Sandwich:
To detect hGalectin-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Galectin-1 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGalectin-1.

Western Blot:
To detect hGalectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

psi-iconProperties
CLONALITY:Polyclonal
CONJUGATE:Biotin
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:Galectin-1 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:LGALS1
ALTERNATE NAMES:GBP, GAL1Galectin-1, 14 kDa laminin-binding protein, Gal-1
ACCESSION NO.:P09382
PROTEIN GI NO.:126155
GENE ID:3956
USER NOTE:Centrifuge vial prior to opening.
psi-iconBackground and References
BACKGROUND:The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin 1 may act as an autocrine negative growth factor that regulates cell proliferation. It also may regulate cell apoptosis and cell differentiation. It binds beta-galactoside, CD45, CD3 and CD4. Galectin 1 inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. It is found in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney, placenta and hematopoietic cells.

ANTIBODIES FOR RESEARCH USE ONLY.

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