Datasheet

GABARAPL2 Antibody
CATALOG NUMBER: 7895

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:GABARAPL2 antibody can be used for detection of GABARAPL2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 13 kDa

Observed: 13 kDa
SPECIFICITY:GABARAPL2 antibody is human, mouse and rat reactive. Multiple isoforms of GABARAPL2 are known to exist.
IMMUNOGEN:GABARAPL2 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human GABARAPL2.

The immunogen is located within the last 50 amino acids of GABARAPL2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:GABARAPL2 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:GABARAPL2 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GABARAPL2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:GABARAPL2 Antibody: ATG8, GEF2, ATG8C, GEF-2, GATE16, GATE-16, FLC3A, Gamma-aminobutyric acid receptor-associated protein-like 2, Ganglioside expression factor 2
ACCESSION NO.:NP_009216
PROTEIN GI NO.:6005768
OFFICIAL SYMBOL:GABARAPL2
GENE ID:11345

Background

BACKGROUND:Gamma-aminobutyric acid (GABA) is the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing in the central nervous system (1). It has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC (2). GABARAPL2 (GABAA receptor-associated protein-like 2), also known as GATE16, was initially identified as a membrane transport modulator and is a mammalian ortholog to the autophagy protein ATG8 (3,4). It is thought that GABARAPL2 and other members of the ATG8 family act as scaffolds for assembly of the Unc-51 like kinase (ULK) complex in the formation of autophagosomes (5).
REFERENCES: 1) Cherubini E, Gaiarsa JL, and Ben-Ari Y. GABA: an excitatory transmitter in early postnatal life. Trends Neurosci.1991; 14:515-19.
2) Dirkx R Jr, Thomas A, Li L, et al. Targeting of the 67 kDa isoform of glutamic acid decarboxylase to intracellular organelles is mediated by its interaction with the NH2-terminal region of the 65 kDa isoform of glutamic acid decarboxylase. J. Biol. Chem. 1995; 270:2241-6.
3) Sagiv Y, Legesse-Miller A, Porat A, et al. GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28. EMBO J. 2000; 19:1494-504.
4) Paz Y, Elazar Z, and Fass D. Structure of GATE-16, membrane transport modulator and mammalian ortholog of autophagocytosis factor Aut7p. J. Biol. Chem. 2000; 275:25445-50.

For Research Use Only