Datasheet

GABARAP Antibody
CATALOG NUMBER: 7359

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Bovine: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:GABARAP antibody can be used for detection of GABARAP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 13kDa

Observed: 15 kDa
SPECIFICITY:Multiple isoforms of GABARAP exist due to alternative splicing events.
IMMUNOGEN:GABARAP antibody was raised against a 19 amino acid peptide near the amino terminus of human GABARAP.

The immunogen is located within the first 50 amino acids of GABARAP.
HOST SPECIES:Rabbit

Properties

PURIFICATION:GABARAP Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:GABARAP Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:GABARAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:GABARAP Antibody: MM46, ATG8A, GABARAP-a, FLC3B, HT004, Gamma-aminobutyric acid receptor-associated protein, MM46
ACCESSION NO.:NP_009209
PROTEIN GI NO.:6005764
OFFICIAL SYMBOL:GABARAP
GENE ID:11337

Background

BACKGROUND:GABARAP Antibody: Gamma-aminobutyric acid (GABA) is the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing in the central nervous system. It has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. GABARAP (GABAA receptor-associated protein) links GABAA receptors to the cytoskeleton and may play a role in intracellular transport of GABAA receptors and its interaction with the cytoskeleton. GABARAP belongs to the MAP1 or ATG8 like family and recent studies show that MAPK15/ERK8 is acting through interaction with ATG8 family proteins to regulate autophagy.
REFERENCES: 1) Cherubini E, Gaiarsa JL, and Ben-Ari Y. GABA: an excitatory transmitter in early postnatal life. Trends Neurosci.1991; 14:515-19.
2) Dirkx R Jr, Thomas A, Li L, et al. Targeting of the 67 kDa isoform of glutamic acid decarboxylase to intracellular organelles is mediated by its interaction with the NH2-terminal region of the 65 kDa isoform of glutamic acid decarboxylase. J. Biol. Chem. 1995; 270:2241-6.
3) Wang H, Bedford FK, Brandon NJ, et al. GABA(A)-receptor-associated protein links GABAA receptors and the cytoskeleton. Nature 1999; 397:69-72.
4) Colecchia D, Strambi A, Sanzone S, et al. MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Autophagy 2012 Sep 4;8(12).

For Research Use Only