Datasheet

FTO Antibody
CATALOG NUMBER: 5137

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:FTO antibody can be used for detection of FTO by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1317 - Human Uterus Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 57 kDa

Observed: 55 kDa
IMMUNOGEN:FTO antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FTO.

The immunogen is located within the first 50 amino acids of FTO.
HOST SPECIES:Rabbit

Properties

PURIFICATION:FTO Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:FTO Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FTO antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:FTO Antibody: ALKBH9, KIAA1752, Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein
ACCESSION NO.:Q9C0B1
PROTEIN GI NO.:148841515
OFFICIAL SYMBOL:FTO
GENE ID:79068

Background

BACKGROUND:FTO Antibody: Rising obesity rates are rapidly becoming a growing health concern in the developing world. The fat mass and obesity associated gene (FTO) is the first gene discovered to contribute to common forms of human obesity. FTO is a member of the non-heme dioxygenase superfamily, encoding a 2-oxoglutarate-dependent nucleic acid demethylase whose mRNA is widely expressed, especially in neurons of feeding-related nuclei of the brain. FTO mRNA in the arcuate nucleus in mice is up-regulated by feeding and down-regulated during fasting, although the opposite pattern has been observed in rats. At least four isoforms of FTO are known to exist.
REFERENCES: 1) Scuteri A, Sanna S, Chen W-M, et al. Genome-wide association scan shows genetic variants in the FTO gene are associated with obesity-related traits. PloS Genet.2007; 3:e115.
2) Gerken T, Girard CA, Tung YCL, et al. The obesity-associated FTO gene encodes a 2-oxyglutarate-dependent nucleic acid demethylase. Science2007; 318:1469-72.
3) Fredriksson R, Hagglund M, Olszewski PK, et al. The obesity gene, FTO, is of ancient origin, upregulated during food deprivation and expressed in neurons of feeding-related nuclei of the brain. Endocrinology2008; 149:2062-71.
4) Stratigopoulous G, Padilla S, Leduc CA, et al. Regulation of FTO/FTM gene expression in mice and humans. Am. J. Physiol. Regul. Integr. Comp. Physiol.2008; 294:R1185-96.

For Research Use Only