| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse, Rat |
| IMMUNOGEN: | FRMPD2 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FRMPD2. The immunogen is located within amino acids 80 - 130 of FRMPD2. |
| TESTED APPLICATIONS: | ELISA, IHC-P, WB |
| APPLICATIONS: | FRMPD2 antibody can be used for detection of FRMPD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. Antibody validated: Western Blot in rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. |
| POSITIVE CONTROL: | 1) Cat. No. 1465 - Rat Kidney Tissue Lysate |
Specifications| PURIFICATION: | FRMPD2 Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | FRMPD2 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | FRMPD2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| OFFICIAL SYMBOL: | FRMPD2 |
| ALTERNATE NAMES: | FRMPD2 Antibody: PDZK4, PDZD5C, PDZK5C, PDZK4, FERM and PDZ domain-containing protein 2, PDZ domain-containing protein 4 |
| ACCESSION NO.: | NP_001018081 |
| PROTEIN GI NO.: | 281604114 |
| GENE ID: | 143162 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | FRMPD2 Antibody: The FERM and PDZ domain containing (FRMPD) protein family consists of four proteins that contain a FERM (Four-point-one, erzin, radixin, moesin) domain and at least one PDZ (PSD-95/Discs large/Zonula-occuldens-1) domain. FRMPD2 also contains an N-terminal KIND domain and three PDZ domains and is structurally similar to the protein tyrosine phosphatase PTP-BL. FRMPD2 is localized in a polarized fashion in epithelial cells at the basolateral membrane and partially co-localizes with the tight-junction marker protein Zonula-occuldens-1. Suppression of FRMPD2 expression via RNAi in Caco-2 cells results in an impairment of tight junction formation, indicating that FRMPD2 plays a major role in tight junction formation. Other experiments indicate that FRMPD2 is a binding partner to several catenin family members and recruitment of FRMPD2 to cell-cell contacts is dependent on E-cadherin-mediated cell-cell adhesion. |
| REFERENCES: | 1) An N, Blumer JB, Bernard ML. The PDZ and Band 4.1 containing protein Frmpd1 regulates the subcellular location of activator of G-protein signaling 3 and its interaction with G-proteins. J. Biol. Chem.2008; 283:24718-28. | 2) Stenzel N, Fetzer CP, Heumann R, et al. PDZ-domain-directed basolateral targeting of the peripheral membrane protein FRMPD2 in epithelial cells. J. Cell Sci.2009; 122:3374-84. | 3) Lee HW, Choi J, Shin H, et al. Preso, a novel PSD-95-interacting FERM and PDZ domain protein that regulates dendritic spine morphogenesis. J. Neurosci.2008; 28:14546-56. | 4) Erdmann KS. The protein tyrosine phosphatase PTP-Basophil/Basophil-like. Interacting proteins and molecular functions. Eur. J. Biochem.270:4789-98. |
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