FRL1 Antibody Cat. No.: 7101

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Rabbit polyclonal FRL1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human FRL1.

The immunogen is located within the last 50 amino acids of FRL1.
TESTED APPLICATIONS: ELISA, IF, WB
APPLICATIONS: FRL1 antibody can be used for detection of FRL1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: FRL1 antibody is human, mouse and rat reactive. Three alternatively spliced transcript variants have been observed
POSITIVE CONTROL:1) Cat. No. 1287 - EL4 Cell Lysate
 2) Cat. No. 17-207 - EL4 Cell Slide
PREDICTED MOLECULAR WEIGHT: 121 kDa

psi-iconProperties
PURIFICATION:FRL1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:FRL1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FRL1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:FMNL1
ALTERNATE NAMES:FRL1 Antibody: FMNL, FHOD4, KW-13, C17orf1, C17orf1B, FMNL, Formin-like protein 1, CLL-associated antigen KW-13
ACCESSION NO.:NP_005883
PROTEIN GI NO.:33356148
GENE ID:752
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:FRL1 Antibody: The Formin-like protein 1 (FRL1) gene encodes a formin-related protein which has been implicated in morphogenesis, cyokinesis, and cell polarity. Formins are a conserved class of proteins expressed in all eukaryotes and have one DAD (diaphanous autoregulatory domain), one FH2 (formin homology 2) domain and one GBD/FH3 (Rho GTPase-binding / formin homology 3) domain. FRL1 is located in the cytoplasm and is highly expressed in the spleen, lymph node and bone marrow cells. FRL1 possibly has a role in the control of cell motility, survival of macrophages and cytoskeletal organization.
REFERENCES:1) Katoh M and Katoh M. Identification and characterization of human FMNL1, FMNL2 and FMNL3 genes in silico. Int. J. Oncol. 2003; 22:1161-8.
2) Yayoshi-Yamamoto S, Taniuchi I and Watanabe T. FRL, a novel formin-related protein, binds to Rac and regulates cell motility and survival of macrophages. Mol. Cell. Biol. 2000; 20: 6872-81.
3) Favaro PM, Traina F, Vassallo J, et al. High expression of FMNL1 protein in T non-Hodgkin’s lymphomas. Leuk. Res. 2006; 30:735-8.
4) Gomez TS, Kumar K, Medeiros RB, et al. Formins regulate the actin-related protein 2/3 complex-independent polarization of the centrosome to the immunological synapse. Immunity 2007; 26:177-90.

ANTIBODIES FOR RESEARCH USE ONLY.

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