Datasheet

FOXRED2 Antibody
CATALOG NUMBER: 6761

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (94%)
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:FOXRED2 antibody can be used for detection of FOXRED2 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1302 - Human Lung Tissue Lysate
IMMUNOGEN:FOXRED2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human FOXRED2.

The immunogen is located within amino acids 70 - 120 of FOXRED2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:FOXRED2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:FOXRED2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FOXRED2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:FOXRED2 Antibody: ERFAD, ERFAD, FAD-dependent oxidoreductase domain-containing protein 2, Endoplasmic reticulum flavoprotein associated with degradation
ACCESSION NO.:No. Q8IWF2
PROTEIN GI NO.:156139139
OFFICIAL SYMBOL:FOXRED2
GENE ID:80020

Background

BACKGROUND:FOXRED2 Antibody: FOXRED2, also known as ERFAD, is an endoplasmic reticulum (ER) luminal flavoprotein which may function in ER associated degradation (ERAD). It has been proposed to bind non-native proteins in the endoplasmic reticulum and target them to the ubiquitination machinery for subsequent degradation. Upon knockdown of FOXRED2, the degradation of the ERAD model substrate ribophorin 332 is delayed, and the overall level of polyubiquitinated cellular proteins is decreased. Defects in the related protein FOXRED1 are a cause of mitochondrial complex I deficiency (MT-C1D), suggesting the FOXRED2 may also play a role in MT-C1D.
REFERENCES: 1) Riemer J, Appenzeller-Herzog C, Johansson L, et al. A luminal flavoprotein in endoplasmic reticulum-associated degradation. Proc. Natl. Acad. Sci. USA 2009; 106:14831-6.
2) Riemer J, Hansen HG, Appenzeller-Herzog C, et al. Identification of the PDI-family member ERp90 as an interaction partner of ERFAD. PLoS One 2011; 6:e17037
3) Calvo SE, Tucker EJ, Compton AG, et al. High-throughput, pooled sequencing identifies mutations in NUBPL and FOXRED1 in human complex I deficiency. Nat. Genet. 2010; 42:851-8.

For Research Use Only