Datasheet

FOXP3 Antibody
CATALOG NUMBER: 7187

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:FOXP3 antibody can be used for detection of FOXP3 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1203 - A549 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 47 kDa
IMMUNOGEN:Rabbit polyclonal FOXP3 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human FOXP3.

The immunogen is located within the last 50 amino acids of FOXP3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:FOXP3 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:FOXP3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FOXP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:FOXP3 Antibody: JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2, Forkhead box protein P3, Scurfin
ACCESSION NO.:NP_054728
PROTEIN GI NO.:31982943
OFFICIAL SYMBOL:FOXP3
GENE ID:50943

Background

BACKGROUND:FOXP3 Antibody: FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators. FOXP3 acts as a repressor of transcription and regulates T cell activation, with its overexpression in CD4 T cells leading to an attenuation of activation-induced cytokine production and proliferation. In regulatory T (Treg) cells, FOXP3 is essential for Treg suppressor function and its expression leads to the repression of IL-17 expression. Genetic mutations involving FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome.
REFERENCES: 1) Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. Nat. Genet. 2001; 27:68-73
2) Schubert LA, Jeffery E, Zhang Y, et al. Scurfin (FOXP3) acts as a repressor of transcription and regulates T cell activation. J. Biol. Chem. 2001; 276:37672-9.
3) Gavin MA, Rasmussen JP, Fontenot JD, et al. Foxp3-dependent programme of regulatory T-cell differentiation. Nature 2007; 445:771-5
4) Kobayashi I, Shiari R, Yamada M, et al. Novel mutations of FOXP3 in two Japanese patients with immune dysregulation, polyendocrinopathy, enteropathy, X linked syndrome (IPEX). J. Med. Genet. 2001; 38:874-6.

For Research Use Only