FOXO1 Antibody Cat. No.: 5811

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%)
IMMUNOGEN: FOXO1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human FOXO1.

The immunogen is located within the first 50 amino acids of FOXO1.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: FOXO1 antibody can be used for detection of FOXO1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide

psi-iconProperties
PURIFICATION:FOXO1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:FOXO1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FOXO1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:FOXO1
ALTERNATE NAMES:FOXO1 Antibody: FKH1, FKHR, FOXO1A, Forkhead box protein O1, Forkhead box protein O1A
ACCESSION NO.:NP_002006
PROTEIN GI NO.:9257222
GENE ID:2308
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:FOXO1 Antibody: FOXO1, also known as FKHR or ForkHead in Rhabdomyosarcoma, is a 70 kDa protein which is a ubiquitously expressed member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. In insulin-responsive tissues, stress or nutrient abundance triggers phosphorylation by PKB/AKT, blocking nuclear translocation and activity. Genetic mutations involving FOXO1A are a cause of alveolar rhabdomyosarcoma (RMS2). Recent studies link the anti-tumor activity of FOXO1 and the process of autophagy.
REFERENCES:1) Anderson MJ, Viars CS, Czekay S, et al. Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics1998; 47:187-99.
2) Greer EL and Brunet A. FOXO transcription factors at the interface between longevity and tumor suppression. Oncogene2005; 24:7410-25.
3) Brunet A, Bonni A, Zigmond MJ, et al. Akt promotes cell survival by phosphorylating and inhibiting a forkhead transcription factor. Cell1999; 96:857-68.
4) Linardic CM. PAX3-FOXO1 fusion gene in rhabdomyosarcoma. Cancer Lett.2008; 270:10-8.

ANTIBODIES FOR RESEARCH USE ONLY.

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