| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Rat: (94%), Mouse: (94%) |
| IMMUNOGEN: | FOXA2 antibody was raised against a 16 amino acid peptide near the center of human FOXA2 . The immunogen is located within amino acids 280 - 330 of FOXA2. |
| TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
| APPLICATIONS: | FOXA2 Antibody can be used for detection of FOXA2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. |
| SPECIFICITY: | At least two isoforms of FOXA2 are known to exist; this antibody will detect both isoforms. FOXA2 antibody is predicted to not cross-react with other members of the FOXA protein family. |
| POSITIVE CONTROL: | 1) Cat. No. 1310 - Human Bladder Tissue Lysate |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 51 kDa Observed: 54 kDa |
Specifications| PURIFICATION: | FOXA2 Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | FOXA2 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | FOXA2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| OFFICIAL SYMBOL: | FOXA2 |
| ALTERNATE NAMES: | FOXA2 Antibody: HNF3B, TCF3B, HNF3B, Hepatocyte nuclear factor 3-beta, Forkhead box protein A2, HNF-3-beta |
| ACCESSION NO.: | NP_068556 |
| PROTEIN GI NO.: | 194394143 |
| GENE ID: | 3170 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | FOXA2 Antibody: FOXA2 is one of three members of the FOXA family, a subset of the forkhead family of transcription factors which play vital roles in development. FOXA2 was initially identified through library screening as a closely related homolog of FOXA1. Both FOXA2 and FOXA1 act as transcriptional activators in adult liver and also play a role in body axis formation, neural tube patterning and definitive endoderm formation during gastrulation. |
| REFERENCES: | 1) Hannenhalli S and Kaestner KH. The evolution of Fox genes and their role in development and disease. Nat. Rev. Genet. 2009; 10:233-40. | 2) Lai E, Prezioso VR, Tao WF, et al. Hepatocyte nuclear factor 3 alpha belongs to a gene family in mammals that is homologous to the Drosophila homeotic gene fork head. Genes Dev. 1991; 5:416-27. | 3) Sasaki H and Hogn BL. Differential expression of multiple fork head related genes during gastrulation and axial pattern formation in the mouse embryo. Dev. 1993; 118:47-59. |
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