FOXA2 Antibody Cat. No.: 7257

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (94%), Mouse: (94%)
IMMUNOGEN: FOXA2 antibody was raised against a 16 amino acid peptide near the center of human FOXA2 .

The immunogen is located within amino acids 280 - 330 of FOXA2.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: FOXA2 Antibody can be used for detection of FOXA2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
SPECIFICITY: At least two isoforms of FOXA2 are known to exist; this antibody will detect both isoforms. FOXA2 antibody is predicted to not cross-react with other members of the FOXA protein family.
POSITIVE CONTROL:1) Cat. No. 1310 - Human Bladder Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 51 kDa

Observed: 54 kDa

psi-iconProperties
PURIFICATION:FOXA2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:FOXA2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FOXA2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:FOXA2
ALTERNATE NAMES:FOXA2 Antibody: HNF3B, TCF3B, HNF3B, Hepatocyte nuclear factor 3-beta, Forkhead box protein A2, HNF-3-beta
ACCESSION NO.:NP_068556
PROTEIN GI NO.:194394143
GENE ID:3170
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:FOXA2 Antibody: FOXA2 is one of three members of the FOXA family, a subset of the forkhead family of transcription factors which play vital roles in development. FOXA2 was initially identified through library screening as a closely related homolog of FOXA1. Both FOXA2 and FOXA1 act as transcriptional activators in adult liver and also play a role in body axis formation, neural tube patterning and definitive endoderm formation during gastrulation.
REFERENCES:1) Hannenhalli S and Kaestner KH. The evolution of Fox genes and their role in development and disease. Nat. Rev. Genet. 2009; 10:233-40.
2) Lai E, Prezioso VR, Tao WF, et al. Hepatocyte nuclear factor 3 alpha belongs to a gene family in mammals that is homologous to the Drosophila homeotic gene fork head. Genes Dev. 1991; 5:416-27.
3) Sasaki H and Hogn BL. Differential expression of multiple fork head related genes during gastrulation and axial pattern formation in the mouse embryo. Dev. 1993; 118:47-59.

ANTIBODIES FOR RESEARCH USE ONLY.

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