Datasheet

FOXA1 Antibody
CATALOG NUMBER: 7255

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (86%), Rat: (79%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:FOXA1 antibody can be used for detection of FOXA1 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
PREDICTED MOLECULAR WEIGHT:52 kDa
SPECIFICITY:Multiple isoforms of FOXA1 exists as a result of alternative splicing event.
IMMUNOGEN:Rabbit polyclonal FOXA1 antibody was raised against a 17 amino acid peptide near the center of human FOXA1.

The immunogen is located within amino acids 270 - 320 of FOXA1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:FOXA1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:FOXA1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FOXA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:FOXA1 Antibody: HNF3A, TCF3A, HNF3A, Hepatocyte nuclear factor 3-alpha, Forkhead box protein A1, HNF-3-alpha
ACCESSION NO.:NP_004487
PROTEIN GI NO.:24497501
OFFICIAL SYMBOL:FOXA1
GENE ID:3169

Background

BACKGROUND:FOXA1 Antibody: FOXA1 is one of three members of the FOXA family, a subset of the forkhead family of transcription factors which play vital roles in development. It has also been implicated in the development of a number of other organs including the androgen and estrogen regulated tissues of the breast and prostate. FOXA1 modulates the transcriptional activity of nuclear hormone receptors and regulates apoptosis by inhibiting the expression of BCL-2. It is an essential protein for the transcriptional activity of both androgen receptor (AR) and estrogen receptor-α (ER). FOXA1 plays a pivotal role from early stage cancer through to drug resistant and metastatic disease. FOXA1 is not only an attractive therapeutic target but could potentially function as a novel biomarker.
REFERENCES: 1) Hannenhalli S and Kaestner KH. The evolution of Fox genes and their role in development and disease. Nat. Rev. Genet. 2009; 10:233-40.
2) Costa RH, Grayson DR, and Darnell JE Jr. Multiple hepatocyte-enriched nuclear factors function in the regulation of transthyretin and alpha 1-antitrypsin genes. Mol. Cell. Biol. 1989; 9:1415-25.
3) Bernardo GM and Keri RA. FOXA1: a transcription factor with parallel functions in development and cancer. Biosci. Rep. 2012; 32:113-30.
4) Robinson JL and Carroll JS. FoxA1 is a key mediator of hormonal response in breast and prostate cancer. Front. Endocrinol 2012; 3:68.

For Research Use Only