Datasheet

FNIP2 Antibody
CATALOG NUMBER: 5045

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:FNIP2 antibody can be used for detection of FNIP2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1467 - Rat Skeletal Muscle Tissue Lysate
SPECIFICITY:Multiple isoforms of FNIP2 are known to exist. This antibody is predicted to not cross-react with FNIP1.
IMMUNOGEN:FNIP2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human FNIP2.

The immunogen is located within amino acids 860 - 910 of FNIP2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:FNIP2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:FNIP2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FNIP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:FNIP2 Antibody: FNIPL, MAPO1, FNIPL, KIAA1450, Folliculin-interacting protein 2, FNIP1-like protein
ACCESSION NO.:NP_065891
PROTEIN GI NO.:154689769
OFFICIAL SYMBOL:FNIP2
GENE ID:57600

Background

BACKGROUND:FNIP2 Antibody: FNIP2 is the second protein found to interact with folliculin, the product of the Birt-Hogg-Dube (BHD) gene. Folliculin is thought to act as a tumor suppressor as mutations or loss of heterozygosity in this gene are associated with BHD syndrome-related renal tumors. Folliculin and FNIP1, a protein that shares 49% identity to FNIP2, bind to AMPK, an important energy sensor in cells that negatively regulates the mammalian target of rapamycin (mTOR), a protein that is thought to be the master switch for cell growth and proliferation. FNIP1 and FNIP2 are able to form homo- and heteromeric multimers, suggesting these proteins may have a functional relationship.
REFERENCES: 1) Hasumi H, Baba M, Hong S-B, et al. Identification and characterization of a novel folliculin-interacting protein FNIP2. Gene2008; 415:60-7.
2) Takagi Y, Kobayashi T, Shiono M, et al. Interaction of folliculin (Birt-Hogg-Dube gene product) with novel Fnip1-like (FnipL/Fnip2) protein. Oncogene2008; 27:5339-47.
3) Vocke CD, Yang Y, Pavlovich CP, et al. High frequency of somatic frameshift BHD mutations in Birt-Hogg-Dube-associated renal tumors. J. Natl. Cancer Inst.2005; 97:931-5.
4) Baba M, Hong SB, Sharma M, et al. Folliculin encoded by the BHD gene interacts with a binding protein, FNIP1, and AMPK, and is involved in AMPK and mTOR signaling. Proc. Natl. Acad. Sci. USA2006; 103:15552-7.

For Research Use Only